Page 215 - Clinical Hematology_ Theory _ Procedures ( PDFDrive )

CHAPTER 9 ■ Leukocytes: Lymphocytes and Plasma Cells 199

TABLE 9.5 Stages of T-Cell Maturation

Double Positive Single Positive

Stages of

Maturation Stem Cell Pro-T Pre-T CD4+ CD8+ (Immature T Cell) Mature T Cell

Anatomic Bone Thymus Thymus Thymus Thymus Peripheral blood

location marrow

Response to None None None Positive and Negative Activation (proliferation

antigen Selection and differentiation)

Surface mem- c-kit+ c-kit+ c-kitn CD4+CD8+ CD4+CD8− CD4−CD8+

brane markers CD44+ CD44+ CD44− TCRCD3 lo TCRCD3 hi TCRCD3 hi

CD25− CD25+ CD25+

Modi ed from Abbas AK, Lichtman AH, Pillai S. Cellular and Molecular Immunology, 8th ed, Philadelphia, PA: Elsevier (Saunders), 2015:191 [Figure 8.12].

Note: Only the stem cell and early Pro-T and mid to late Pre-T lymphocytes can proliferate. Expression of RAG occurs in the Pro-T, very early Pre-T, and in the
early to mid stage of CD4+CD8+ development. TdT expression occurs in most of the Pro-T stage and extremely early in the Pre-T phase.

m x m Monoclonal Antibodies

- x (ADCC) U m , m k

– x CD z :

. B m , CD m m -

- k m mm . S m m k

(IFN-γ), GM-CSF, IL- , IL- , m ( . . ).

mm . F , - . S m m k x ,

mm (IFN-γ) NK- m .

k , T mm , - F x m , CD

APC MHC I x , m , m m

m k , CD +, CD +, CD −; m x m -

m - - CD +, CD −, CD +. I

m . CD

TABLE 9.6 Stages of B-Cell Maturation

Stages of B-Cell

Maturation Stem Cell Pro-B Pre-B Immature B Mature B

Anatomic location Bone Bone Bone marrow Bone marrow/ Peripheral blood

marrow marrow peripheral blood

Response to None None None Negative selection (deletion), Activation (proliferation and
antigen receptor editing differentiation)

Surface membrane CD43+ CD43+ B220 lo IgM lo IgM hi

markers CD19+ CD43+ CD43 (neg) CD43 (neg)

CD10+

Immunoglobulin None None Cytoplasmic µ and Membrane IgM (µ+ kappa or Membrane IgM and IgD
expression pre-B receptor- lambda light chain)

associated µ

Modi ed from Abbas AK, Lichtman AH, Pillai S. Cellular and Molecular Immunology, 8th ed, Philadelphia, PA: Elsevier (Saunders), 2015:185 and 191 [Figure 8.12].

Note: Only the stem cell and early Pro-B lymphocytes can proliferate. Expression of RAG occurs in the late Pro-B/Pre-B stages of development and in the early

immature B phase of development.