Page 335 - Clinical Hematology_ Theory _ Procedures ( PDFDrive )

CHAPTER 17 ■ Hemoglobinopathies and Thalassemias 319

D m m - m m

, m m m - DNA m (PCR).

, m ,

. Prenatal Diagnosis

P m m m
Hem oglobin Electrophoresis q SCD. A DNA m

A , L P - m , m

m m SCD m k . T -

m (F . . ). T m DNA

k m - m k .

m q ,

k m . Screening of New borns for Sickle Cell Disease

I SCD, m % % and Carriers

H S, % % H F, m m H A . T m H F. T -

% H F % H A m m .
High-Pressure Liquid Chrom atography H F m α- γ- . D

E k H m , β- -

m - x HPLC m γ- . I m m

m q H A H F , x m % -α γ-

m . T m m- % β- .

m - - S SCD m

m m m D C m . SCD (H SS)

. A Am U

S m
DNA Analysis . W m -

W - m j -

m , m m , SCD

m m . T - m . E

m SCD

DNA . k

T . A m m

m m , , m j

, . V SCD .

S x m k -

SCD. A k

m β β S ,

m H F H A H S.

T m H A H S

α m β .

S m

. A

k m -

, m

. C m

m .

B -

m m ,

. A m

, m
FIGURE 17.6 H m m m . B m k

– H . . Le lane. A m x H C, m

H S, H F. Middle lane. C m k

m H S H F, H A ( m m m ( . ., -
H S–β m H S HBFH). Right lane. k , , m ). S m

C m k H S, H F, m k m

H A. (R m M C KD. Clinical Laboratory k m m . S m m
Medicine, , P , PA: L W m & W k , -

, m .) - .