Page 410 - Clinical Hematology_ Theory _ Procedures ( PDFDrive )

394 PART 6 ■ Neoplastic Disorders

. A m -

m m k m . TABLE 21.9 Sudan Black B Reactions

Basophilic Leukem ia
Cell Type Reaction
B k m (m k m ) m

k m . F q , m Positive reactions

m k . P m Granulocytic cells Become increasingly posi-

k m x k , k - (neutrophils and tive (sudanophilic) as they

x × /L. P m eosinophils) mature

m % .
Myeloblasts May have a few, small gran-

ules in the Golgi region
Acute Megakaryoblastic Leukemia FAB M7
Promyelocyte Increased granulation
T WHO m megakaryoblastic leukemia. I Neutrophilic myelocytes Granules concentrated near

m k m , % m the nucleus or rim of the

m k (F . . ). A m k - cytoplasm

k m . I

x m % % AML. Metamyelocytes, Strongly positive
bands, and segmented

Clinical Signs and Sym ptom s neutrophils

O m q x . R Eosinophils at all stages Granules react positively at

. the periphery of the granule

Laboratory Data Monocytes and May have granules scattered
precursors over the entire cell
C , m .

D m Variable reactions

. T q m m m - Basophils

m AML. Imm Negative reactions

m k x m (sudanophobic)

: CD CD . B - Lymphocytes and

MPO . lymphocytic precursors

P , .
Megakaryocytes and

thrombocytes (platelets)
NOTE: This is a good time to complete Review Questions

related to the preceding content. Erythrocytes
Erythroblasts may dis-

play a few granules that
Cytochemical Staining represent mitochondrial

V m phospholipid components

m . A m mm

, m m ( TABLE 21.10 Myeloperoxidase Reactions

. – . , B x . ).

Cell Type Reaction

Positive reactions

Neutrophilic granulocytes Strongly positive

except blast forms

Eosinophils Positive

Monocytes except blast Positive, but reaction is faint

forms with few granules

Negative reactions

Basophils

Lymphocytic cell series

FIGURE 21.15 A k m M . (R m M C Plasma cell series

KD. Clinical Laboratory Medicine, , P , PA:

L W m & W k , , m .) Erythrocytic cell series