Page 102 - APS(2022)_TG5 _Edisi Guru

Sains Tingkatan 5 Bab 1
Tarikh:

EKSPERIMEN Kesan Antibiotik terhadap Pertumbuhan Bakteria 1
Wajib 1.10 Effect of Antibiotics on Bacterial Growth
Pendekatan inkuiri
BAB
PEKA
Tujuan Mengkaji kesan kepekatan antibiotik terhadap pertumbuhan bakteria.
Aim To study the effect of antibiotic concentration on bacterial growth. K1P1

Pernyataan Adakah kepekatan antibiotik yang berbeza mempengaruhi pertumbuhan bakteria?
Masalah Do different concentration of an antibiotic affect the bacterial growth?
Problem Statement
Hipotesis Semakin tinggi kepekatan antibiotik, semakin luas kawasan jernih di atas permukaan
Hypothesis agar-agar nutrien. EKSPERIMEN WAJIB
The higher the concentration of an antibiotic, the larger the clear area on the surface of the nutrient agar. K1P2
Kepekatan antibiotik
Pemboleh Ubah (a) Dimanipulasikan :
Variables
Manipulated The concentration of the antibiotic

Keluasan kawasan jernih
(b) Bergerak balas :
Responding The size of the clear area

(c) Dimalarkan : Jenis antibiotik, jenis bakteria, isi padu bakteria yang digunakan
Constant The type of antibiotic, the type of bacteria, the volume of bacteria used K1P3

Bahan Agar nutrien steril, kultur bakteria Bacillus subtilis
Materials Sterile nutrient agar, cultures of bacteria Bacillus subtilis
Radas Piring petri steril, pita selofan, picagari 1 cm , cakera antibiotik berkepekatan tinggi, cakera
3
Apparatus antibiotik berkepekatan rendah dan forseps
Sterile petri dish, cellophane tape, 1 cm syringe, antibiotic disc with a high concentration, antibiotic disc with a low
3
concentration and forceps K1P4
Prosedur
Procedure
Cakera antibiotik berkepekatan rendah Agar nutrien + kultur bakteria
K2 Antibiotic disc with a low concentration Nutrient agar + bacterial culture
PI
1
2 Piring Petri Cakera antibiotik berkepekatan tinggi
3 Petri dish Antibiotic disc with a high concentration
4
S
1. Sediakan susunan radas seperti yang ditunjukkan dalam rajah.
Set up the apparatus as shown in the diagram.
3
3
2. Isi piring Petri dengan 5 cm agar nutrien steril dan 1 cm kultur bakteria.
3
Fill a Petri dish with 5 cm of sterile nutrient agar and 1 cm of bacterial culture.
3
3. Letakkan cakera antibiotik berkepekatan tinggi dan cakera antibiotik berkepekatan rendah
di atas permukaan agar nutrien.
Place an antibiotic disc with a high concentration and another antibiotic disc with a low concentration onto the
surface of the nutrient agar.
4. Tutup piring Petri dengan penutup dan lekatkan penutup itu menggunakan pita selofan.
Cover the Petri dish with a lid and seal the lid using cellophane tape.
5. Terbalikkan piring Petri dan biarkan selama dua hari dalam suhu bilik.
Invert the Petri dish and leave it aside for two days at room temperature.
6. Rekod dan lukiskan rajah untuk menunjukkan keadaan agar nutrien pada akhir eksperimen.
Record and draw a diagram to show the condition of the nutrient agar at the end of the experiment.
Kaedah 1. Susunan radas sediakan seperti yang ditunjukkan dalam rajah.
Method
The apparatus was set up as shown in the diagram.

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