Page 14 - REC :: M.Tech. BioTech Curriculum and Syllabus - R2019

biomass and product formation – Maintenance coefficients – Oxygen consumption and heat evolution in aerobic
cultures – Thermodynamic efficiency of growth.
UNIT-II MICROBIAL GROWTH, KINETICS, MAINTENANCE AND PRODUCT FORMATION 9
Phases of cell growth in batch cultures – Simple unstructured kinetic models for microbial growth – Substrate
utilization and product formation – Growth associated and non-growth associated product formation kinetics –
Monod and Leudeking-Piret models – Effects of inhibition – Determination of kinetic parameters by batch, fed
batch and continuous culture and analysis of chemo state performance – Role of maintenance and endogenous
metabolism in substrate utilization andgrowth.
UNIT-III STRUCTUREDMODELS 9
Structured models for growth and product formation – Compartmental and metabolic models , Chemically and
genetically structured models – Kinetics of growth and product formation by filamentous organisms –
Considerations for the production of r-DNAproducts.
UNIT-IV MASS TRANSFER INBIOLOGICALSYSTEMS 9
Interphase Gas-Liquid mass transfer – General oxygen balances for Gas-Liquid transfer – Volumetric mass
transfer co-efficient – Models for oxygen transfer in large scale bioreactors – Case studies for large scale
bioreactors – Model for oxygen gradients in air lift bioreactor

UNIT-V DIFFUSION AND BIOLOGICAL REACTION IN IMMOBILIZED BIOCATALYST 9
SYSTEMS
External mass transfer – Internal diffusion and reaction within biocatalysts – Derivation of finite difference
model for diffusion – Reaction systems – Dimensionless parameters from diffusion – Reaction models –
Effectiveness factor concept – Case study for diffusion with biological reaction
Total Contact Hours : 45

Course Outcomes:
 The students will learn about the stoichiometry and balances of substrate andbiomass.
 The students will learn how to find the different modes of cultivation parameters and itskinetics.
 The students will gain knowledge about the various structured kinetic models and its application techniques.
 The students will gain knowledge about the practical problems arising on the performance of bioreactors.
 The student will learn to work on immobilized bedbioreactors.

Text Book(s):
nd
Dunn, I.J., Heinzle, E., Ingham, J. and Prenosil, J.E., “Biological Reaction Engineering”, 2 Edition, WILEY-
1
VCH publications,2003.
2 Dutta, R., “Fundamentals of Biochemical Engineering”, Springer,2008.
3 Shuler, M.L. and Kargi, F., Bioprocess Engineering: Basic concepts, 2 Ed., Prentice-Hall,2002.
nd
nd
4 Doran Pauline M, Bioprocess Engineering Principles, 2 Ed., Academic Press,1995.
nd
5 Nielsen,J.andVilladsen,J.,BioreactionEngineeringPrinciples,2 Ed.,Springer,2007.
nd
6 Blanch,H.WandClarkD.S.,BiochemicalEngineering,2 Ed., MarcelDekker,1997.

Reference Books(s) / Web links:
1 Najafpour, G.D., “Biochemical Engineering & Biotechnology”, Elsevier,2007.
Truskey, G.A., Yuan, F. and Katz, D.F., “Transport Phenomena in Biological Systems”, Pearson Prentice
2
Hall,2004.
Katoh, S. and Yoshida, F., “Biochemical Engineering – A Text Book for Engineers, Chemists and Biologists”,
3
Wiley publications,2009.

BY19203 BIOPHARMACEUTICALS AND BIOSIMILARS Category L T P C
3 0 0 3

Objectives:
 To provide knowledge on drug development approval process.
 To impart knowledge in advanced molecular concepts in Biosimilar production