Page 57 - B.Tech Biotech Curriculum and Syllabus R2017 - REC

Department of BIOTECHNOLOGY, REC

UNIT IV ORGANIZATION AND STRUCTURE OF GENOMES 9
Organization and structure of genomes, Genome sequencing methods, Conventional and shotgun genome
sequencing methods, Next generation sequencing technologies. Genetic maps and Physicalmaps,Restriction
Enzyme Finger Printing, Hybridization mapping, Radiation Hybrid Maps, Optical mapping. ORF finding and
functional annotation.

UNIT V CURRENT STATUS OF GENOME SEQUENCING PROJECTS 9
Current status of genome sequencing projects, Introduction to Functional genomics, Microarrays, Serial
Analysis of Gene expression (SAGE), Subtractive hybridization, DIGE, TOGA, Yeast Two hybrid System.

TOTAL:45 PERIODS
OUTCOMES:
On completion of this course
 The students will be aware of how to clone commercially important genes.
 The students will be able to produce the commercially important recombinant proteins.
 The students will be aware of gene and genome sequencing techniques.
 The students will gain knowledge about microarrays,
 The students will be able to analyse of Gene expression and proteomics.

TEXT BOOKS:
1. Primrose SB and R. Twyman ―Principles Of Gene Manipulation &GeneomicBlackwell Science
Publications, 2006.
2. Principles of Genome Analysis and Genomics by S.B.Primrose and R.M.Twyman, Third Edition
(Blackwell Publishing), 2003.
3. Gene cloning and DNA analysis by T.A Brown ,Sixth edition

REFERENCES:
1. Ansubel FM, Brent R, Kingston RE, Moore DD, ―Current Protocols In Molecular Biology ―Greene
PublishingAssociates, NY, 1988.
2. Berger Sl, KimmerAR, ―Methods In Enzymology‖, Vol 152,Academic Press, 1987.
3. Genomes 3 by T.A.Brown, Third Edition (Garland Science Publishing)

BT17604 CHEMICAL REACTION ENGINEERING L T P C
3 0 0 3

OBJECTIVES:
 To impart the knowledge of reaction rate theories and reaction mechanisms to derive expressions for
rate equations mass and energy balances.
 To provide a core foundation for the analysis and design of chemical reactors.

UNIT I SCOPE OF CHEMICAL KINETICS & CHEMICAL REACTION ENGINEERING
9
Broad outline of chemical reactors; rate equations; concentration and temperature dependence; development
of rate equations for different homogeneous reactions.

UNIT II IDEAL REACTORS 9
Isothermal, batch, flow, semi-batch reactors; performance equations for single reactors; multiple reactor
systems.
UNIT III IDEAL FLOW AND NON IDEAL FLOW 9
RTD in non-ideal flow; non-ideal flow models; reactor performance with non-ideal flow.

UNIT IV GAS-SOLID, GAS-LIQUID REACTIONS 9
Introduction – nature of catalyst, catalyst preparation - rate equation for surface kinetics; pore diffusion;
effectiveness factor, thiele modulus; heat effects during reaction; progressive conversion module and
shrinking core model.
Curriculum and Syllabus | B.Tech. Biotechnology | R2017 Page 57