Page 56 - B.Tech Biotech Curriculum and Syllabus R2017 - REC

Department of BIOTECHNOLOGY, REC

UNIT V RECOMBINANT CELL CULTIVATION 7
Different host vector system for recombinant cell cultivation strategies and advantages. E.coli, yeast Pichia
pastoris/ Saccharomyces cereviseae, Animal cell cultivation, plant cell cultivation, Insect cell cultivation.
High cell density cultivation, process strategies, reactor considerations in the above system

TOTAL: 45 PERIODS

COURSE OUTCOMES:
The students will learn
 Design of different types of reactors like Chemostat, packed bed, fluidized bed, airlift and bubble
column reactors.
 Scale up reactors
 Design of immobilised enzyme bioreactors
 Analyze, develop and simulate bioprocess models.
 Knowledge in recombinant cell cultivation like animal cell, plant cell, insect cell and high cell density
cultivation.

TEXT BOOKS
1. Shuler, Michael L. and Fikret Kargi, ―Bioprocess Engineering ―, Prentice Hall, 1992.
2. Doran M Pauline ―Bioprocess Engineering Principles‖. 2 nd Edition, Elsevier, 2012.
3. Ghasem D.Najafpour, ―Biochemical Engineering and Biotechnology‖, Elsevier, 2007.

REFERENCES
1. Anton Moser, ―Bioprocess Technology, Kinetics and Reactors‖, , Springer Verlag.
2. James E. Bailey & David F. Ollis, Biochemical Engineering Fundamentals, McGraw Hill.
3. James M. Lee, Biochemical Engineering, PHI, USA.
4. Atkinson, Handbook of Bioreactors,Harvey W. Blanch, Douglas S. Clark, Biochemical Engineering,
Marcel Decker Inc.
5. Shuler and Kargi, ―Bioprocess Engineering ―, Prentice Hall, 1992.
6. Pauline Doran, Bioprocess Engineering Calculation, Blackwell Scientific Publications. Harvey W. Blanch,
Douglas S. Clark, Biochemical Engineering, Marcel Dekker, Inc

BT17603 GENETIC ENGINEERING L T P C
4 0 0 4
OBJECTIVES:
 To discuss the gene cloning methods and the tools and techniques involved in gene cloning and
genome analysis and genomics.
 To explain the heterologous expression of cloned genes in different hosts, production of recombinant
proteins and PCR techniques.
 To explain comparative genomics and proteomics.

UNIT I BASICS OF RECOMBINANT DNA TECHNOLOGY 9
DNA Manipulative enzymes, Linkers and Adaptors. Characteristics of cloning and expression vectors based
on plasmid and bacteriophage, Vectors for yeast, insect and mammalian systems, Prokaryotic and eukaryotic
expression host systems, Introduction of recombinant DNA in to host cells and selection methods.

UNIT II DNA LIBRARIES 9
Construction of genomic and cDNA libraries, Artificial chromosomes – BACs and YACs, Chromosome
walking, Screening of DNA libraries using nucleic acid probes and antisera.

UNIT III SEQUENCING AND AMPLIFICATION OF DNA 9
Maxam Gilbert‘s and Sanger Coulson‘s and automated methods of DNA sequencing, Inverse PCR, Nested
PCR, AFLP-PCR, Allele specific PCR, Assembly PCR, Asymmetric PCR, Touch down PCR, Hot start PCR,
Colony PCR, single cell PCR, Real-time PCR/qPCR – SYBR green assay, Taqman assay, Molecular beacons,
Site directed mutagenesis.

Curriculum and Syllabus | B.Tech. Biotechnology | R2017 Page 56