Page 115 - 2022-23 BIOFACT Catalogue

DNA / RNA Amplification Other Reagent

- Comparison of gradient PCR efficiency on various concentrations of 5X Band Helper™
5X Band Helper™
BH 0X BH 0X
DRD4 SOX (1 kb)
M 1 2 3 4 5 6 7 8 9 10 11 12 M M 1 2 3 4 5 6 7 8 9 10 11 12 M

BH 1X BH 1.5X
DRD4 SOX (1 kb)
M 1 2 3 4 5 6 7 8 9 10 11 12 M M 1 2 3 4 5 6 7 8 9 10 11 12 M

Template : Human genomic DNA 10 ng PCR Condition : 35 cycles M : 1kb DNA Ladder
1: 50.0℃ 2: 50.3℃ 3: 51.2℃ 4: 52.7℃ 5: 54.7℃ 6: 57.2℃ 7: 60.2℃ 8: 62.9℃ 9: 64.9℃ 10: 66.6℃ 11: 67.5℃ 12: 68.0℃

※ PCR was performed using BioFACT™ Taq DNA Polymerase.

Set-up of 5X Band Helper™
Please perform PCR ampli cation by adjusting the nal concentration of 5X Band Helper™ from 0.5X to 2X and choose the optimal
concentration of 5X Band Helper™.

How to use Band Helper™ Product Information
5X Band Helper™ [BB741-10k]
Reaction mixture Mix Ⅰ Mix Ⅱ Mix Ⅲ Mix Ⅳ Mix Ⅴ
(conc. of 5X BandHelper™) (0X) (0.5X) (1X) (1.5X) (2X)
10X Taq Reaction Bu er 5 ㎕ 5 ㎕ 5 ㎕ 5 ㎕ 5 ㎕
Each 10 mM dNTP mix 1 ㎕ 1 ㎕ 1 ㎕ 1 ㎕ 1 ㎕
Primer F (10 pmole /㎕) 2 ㎕ 2 ㎕ 2 ㎕ 2 ㎕ 2 ㎕
Primer R (10 pmole /㎕) 2 ㎕ 2 ㎕ 2 ㎕ 2 ㎕ 2 ㎕
Template DNA - ㎕ - ㎕ - ㎕ - ㎕ - ㎕
5X Band Helper™ 0 ㎕ 5 ㎕ 10 ㎕ 15 ㎕ 20 ㎕
Taq (5 U/㎕) 0.25 ㎕ 0.25 ㎕ 0.25 ㎕ 0.25 ㎕ 0.25 ㎕ ▶Contents ▶Expiration date
Add D.W to 50 ㎕ 50 ㎕ 50 ㎕ 50 ㎕ 50 ㎕ 5X Band Helper™ 2 Year and 3 months at -20 ± 5℃

Maker Cat.No. Product Size
BIOFACT BB741-10k 5X Band Helper™ 1 ㎖ × 10 ea

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BIOFACT CATALOGUE