Page 116 - 2022-23 BIOFACT Catalogue

BioFACT™ RNase Inhibitor

E cient Protection of RNA from Ribonuclease

BioFACT™ RNase Inhibitor is a 91 KDa recombinant protein derived from Rattus norvegicus. It inhibits RNase activity through a 1:1
non-covalent binding with RNase A, B and C, and does not a ect the activity of RNase H, T1, S1 nuclease, SP6 RNA polymerase, T7 RNA
polymerase, T3 RNA polymerase, AMV reverse transcriptase, M-MLV Reverse transcriptase, and Taq DNA polymerase.
BioFACT™ RNase Inhibitor is useful for protecting RNA from ribonuclease during cDNA synthesis, one-step RT-PCR, RNA synthesis, and
RNA preparation experiments.

Feature
• 1 Unit : Activity that inhibits 50% of the activity of 5 ng of RNase A • Molecular weight : 91 kDa Protein
• Source : An E.coli strain with a recombinant RNase Inhibitor gene • Heat inactivation : 70 ℃ for 10 min
from Rattus • Optimal pH range : pH 5.5 ~ 9.0

Application
• Isolation and puri cation of RNA • In vitro transcription/translation reactions
• RT-PCR reactions • RNase protection assays
• cDNA synthesis reactions • RNA-dependent in vitro functional assays

QC DATA
- Comparison of LOD between BioFACT™ RNase Inhibitor and other company’s RNase Inhibitor
(Concentration of RNase A is fixed to 1.6 ng)

Company A Company A
M 1 2 3 4 5 6 M M 1 2 3 4 5 6 M

BIOFACT BIOFACT
M 1 2 3 4 5 6 M M 1 2 3 4 5 6 M

RNase Inhibitor RNase A
1 : 4 unit 2 : 2 unit 3 : 1 unit 4 : 0.5 unit 5 : 0.25 unit 6 : 0.13 unit 1 : 1600 pg 2 : 800 pg 3 : 400 pg 4 : 200 pg 5 : 100 pg 6 : 50 pg

116 BIOFACT CATALOGUE