Page 163 - 2022-23 BIOFACT Catalogue

DNA / RNA Preparation

QC DATA
- Comparison of extraction and PCR amplification efficiency between HiGene™ Genomic DNA Prep Kit for Soil
and other company’s prep kit
<Genomic DNA extraction efficiency on various soil>
Company A BIOFACT
M 1 1 2 2 3 3 4 4 5 5 6 6 7 7 8 8 M 1 1 2 2 3 3 4 4 5 5 6 6 7 7 8 8

Sample vol. : 500mg 1 : Mountain soil 2 : Paddy soil 3 : Field (Acre) soil 4 : Fertilizer soil 5 : Soil, Upstream of the river
6 : Soll, Downstream of the river 7 : Soil, Riverside 8 : Soil, Mud flat
M : 1kb DNA Ladder 1% Agarose gel 3㎕ loading Elution volume : 100 ㎕

< PCR amplification efficiency on various soil>
A : Company A B : BIOFACT (Grinder) C : BIOFACT (Genie 2 vortex) A : Company A B : BIOFACT (Grinder) C : BIOFACT (Genie 2 vortex)
1 2 3 M 1 2 3 M 1 2 3 1 2 3 M 1 2 3 M 1 2 3

Soil,
Mountain soil Upstream
of the river

Soil,
Paddy soil Downstream
of the river

Field (Acre) soil Soil,
Riverside

Fertilizer soil Soil,
Mud flat

A : Company A BIOFACT (Grinder, GeneReady 5m/s, 40sec) BIOFACT (Vortexer, Genie 2 9-10 level at max. strength (600-3200 rpm), 5 mins.)
Genomic DNA Conc. 1 : 5 0 2 : 5 -1 3 : 5 -2 Target Gene (size) : 16s rRNA (1.5kb) M : 1kb DNA Ladder 1% Agarose gel 3㎕ loading Elution volume : 100 ㎕

Conclusion
In PCR amplification, it can be confirmed that genomic DNA was amplified from its undiluted
concentration as the effect of inhibition is less than that of other kit.

- Comparison of extraction time between HiGene™ Genomic DNA Prep Kit for Soil and other company’s prep kit

BIOFACT 25 mins.

Company A 42 mins.

(mins.) 0 10 20 30 40 50

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BIOFACT CATALOGUE