Page 165 - 2022-23 BIOFACT Catalogue

Column Type
Solution Type DNA / RNA Preparation
Magnetic Bead Type
Easy Type

HiGene™ Genomic DNA
Magnetic Solution Column Easy
Bead Type Type Type Type
Prep Kit [Solution Type]

High Yield Genomic DNA Preparation from Small Sample Volume

HiGene™ Genomic DNA Prep Kit [Solution Type] is a safe system for isolation of high yield and high quality genomic DNA by salt-
ing-out method, not using existing phenol-chloroform method. This system allows easy scale up and minimizes the DNA fragmenta-
tion which is the problem caused by spin column typed kits or other ltration typed kits.
Feature
• Solution type • High yield
• Easy scale up • Safe and cost-saving kit

Application
• PCR (Real-Time PCR, Multiplex PCR, Long PCR and etc.) • Library construction
• Southern blotting analysis • Genotyping (SNP, SSR, AFLP, T-RFLP and etc.)

Workflow

- HiGene™ Genomic DNA Prep Kit For Bacterium [GD262-060]

Mixing sample + Protein
+ Cell Lysis Solution Precipitation Ethanol down Washing DNA elution
+ RNase A Solution

QC DATA

- Comparison of gDNA isolation efficiency between HiGene™ Genomic DNA Prep Kit [Solution Type] and
other company's prep kit
<Animal Tissue - Bovine Tissue, 10 mg>
BIOFACT Company A
M M
Bovine Sample Conc. OD(260/280) Total DNA conc.
1 97.9 ng/㎕ 9.7 ㎍
BIOFACT 1.91
2 99.9 ng/㎕ 9.9 ㎍
1 57.0 ng/㎕ 5.7 ㎍
Company A 1.94
2 54.3 ng/㎕ 5.4 ㎍
M : 1kb DNA Ladder 1% Agarose gel 3㎕ loading Elution volume : 100 ㎕

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BIOFACT CATALOGUE