Page 167 - 2022-23 BIOFACT Catalogue

DNA / RNA Preparation

<Human - Tissue Cultured Cell>
M BIOFACT Company A M
Tissue Cultured Sample Conc. OD(260/280) Total DNA conc.
Cell
1 322.1 ng/㎕ 32.2 ㎍
BIOFACT 1.99
2 385.3 ng/㎕ 38.5 ㎍
1 301.4 ng/㎕ 30.1 ㎍
Company A 2.00
2 312.4 ng/㎕ 31.2 ㎍
M : 1kb DNA Ladder 1% Agarose gel 3㎕ loading Elution volume : 100 ㎕

<Human - Whole Blood>
M BIOFACT Company A M
Whole Blood Sample Conc. OD(260/280) Total DNA conc.
1 60.8 ng/㎕ 6.1 ㎍
BIOFACT 1.84
2 64.4 ng/㎕ 6.4 ㎍
1 27.4 ng/㎕ 2.7 ㎍
Company A 1.76
2 21.6 ng/㎕ 2.1 ㎍
M : 1kb DNA Ladder 1% Agarose gel 3㎕ loading Elution volume : 100 ㎕

<Human - Saliva>
M BIOFACT Company A M
Saliva Sample Conc. OD(260/280) Total DNA conc.
1 134.8 ng/㎕ 13.5 ㎍
BIOFACT 1.92
2 113.7 ng/㎕ 11.4 ㎍
1 16.9 ng/㎕ 1.7 ㎍
Company A 2.05
2 17.1 ng/㎕ 1.7 ㎍
M : 1kb DNA Ladder 1% Agarose gel 3㎕ loading Elution volume : 100 ㎕

Know-how for high yield
1. Please use recommended volume / amount of each sample. In case of isolation from large amount of sample, adjust the input
volume of each solution in proportion to the amount of sample.
2. Prepare 80% Ethanol (not provided) in a WB Bottle. Make it fresh before use.
3. Please remove EtOH completely thorough column centrifugation before using DNA Hydration Solution.
4. Add dried enzyme to D.W (Bu er) and mix well. Please store it at -20°C.
5. Please use the product according to its expiration date.
6. Please do not vortexing strongly to avoid DNA degradation during cell lysis step.
7. Storage of Cell lysis solution at lower temperature may cause precipitation of salts due to SDS.
In this case, please use it after thawing in the microwave/dry oven.

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BIOFACT CATALOGUE