Page 172 - 2022-23 BIOFACT Catalogue
P. 172

- DaBead™ Total RNA Prep Kit Prep Kit _ APX-32P  [RP801A-096]
                                                                                        Sample  Magnetic  DNA
                                                                                            Bead




                       1  2  3  4  5  6  1  2  3  4  5  6  1  2  3  4  5  6  1  2  3  4  5  6  1  2  3  4  5  6
                        Bead binding     Lysis bu er    Washing bu er1,2  Elution bu er   Release bead



                   - DaBead™ Total RNA Prep Kit _ 96well Press Pipettor [RP901-096, RP401-096]








                     Cell Lysis &      DNA binding              Washing               DNA elution
                    precipitation



                  QC DATA

                  - Comparison of extraction efficiency on various plant samples
                      Lactuca sativa (Leaf)   Brassica napus (Stem)   Ixeridium dentatum (Root)  Perilla frutescens (Seed)
                   M  BIOFACT     Company A  M  BIOFACT    Company A  M  BIOFACT     Company A  M  BIOFACT     Company A








                                                       M : 1kb DNA Ladder                1% Agarose gel            3㎕ loading              Elution volume : 100 ㎕


                  - RT-PCR LOD test (PCR inhibition) on various concentrations   - qRT-PCR LOD Test (PCR inhibition)

                   Lactuca sativa   Brassica napus   Ixeridium dentatum   Perilla frutescens
                     (Leaf)    (Stem)    (Root)    (Seed)      Lactuca sativa   Brassica napus   Ixeridium dentatum   Perilla frutescens
                                                                 (Leaf)    (Stem)    (Root)    (Seed)
                  M   1     2    3     4      M   1     2    3     4      M   1     2    3     4      M   1     2    3     4





                  M : 1kb DNA Ladder       1 : Undiluted(5 0 )       2 : 5 -1        3 : 5 -2        4 : 5 -3        Target Gene : 18S rRNA  Template : Undiluted(5 0 ) , 5 -1  , 5 -2  , 5 -3          Target Gene : 18S rRNA
                ** As a result of the Template LOD test, PCR can be performed without PCR Inhibition (High Purity)   ** As a result of the Template LOD test, PCR can be performed without PCR Inhibition (High Purity)







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