Page 180 - 2022-23 BIOFACT Catalogue

Column Type
Solution Type
Magnetic Bead Type
Easy Type
HiGene™

Magnetic Solution Column Easy
Bead Type Type Plasmid Mini Prep Kit (Ver.2.0)
Type
Type
Convenient and Reliable Isolation of High Quality Plasmid DNA in 2 Steps

HiGene™ Plasmid Mini Prep Kit (Ver. 2.0) is a upgraded kit for time-saving and convenient use. Isolation procedure has been reduced and
simpli ed in two steps through application of alkaline lysis method and reduction of clearing time of lysate. HiGene™ Plasmid Mini Prep Kit
(Ver. 2.0) is designed to isolate over 20㎍ high purity plasmids as a spin column type and it is suitable for isolation from low copy plasmid to
large sized circular DNA such as fosmid and BAC clone. Isolated plasmid DNA is applicable for various molecular biological applications such
as PCR, cloning, general sequencing and in vitro transcription. Included, Blue indicator, is a pH indicator which helps users visually check the
pH changes during separation process for plasmids.

Feature
• Spin column type • Sequencing grade
• Membrane : Glass microfiber • Low copy plasmid prep
• Column binding capacity : 20 ㎍ DNA / column • Large size circular DNA (Fosmid, BAC clone) prep

Workflow
- HiGene™ Plasmid Mini Prep Kit [PM105-100]

Cell resuspension & Neutralization Column binding & Elute plasmid Pure plasmid DNA
lysis (Blue Indicator : Blue) (Blue Indicator : Colorless) washing column DNA

QC DATA
- Comparison of isolation efficiency on various plasmid sizes between HiGene™ Plasmid Mini Prep Kit (Ver.2.0)
and other companies' prep kits
Company A BIOFACT
A사
BIOFACT사
M Company A Company B Company C BIOFACT M Company A Company B Company C BIOFACT M 1 2 3 1 2 3 M
B사
BIOFACT사
C사
A사
C사
BIOFACT사
A사
B사
pET21b pMW118 Fosmid
M : 1Kb DNA ladder 1% agarose Gel 5 ㎕ loading 1 : Fosmid + insert 14kb Prep
M : 1kb DNA Ladder 1% Agarose gel 3㎕ loading Elution volume : 100 ㎕
1 : Fosmid + insert 14kb Prep
G : G사 Q : Q사 I : I사 B : BIOFACT 2 : Fosmid
2 : Fosmid
3 : Fosmid + insert 14kb DNA digestion
3 : Fosmid + insert 14kb DNA digestion
180 BIOFACT CATALOGUE

M 1 2 3 4 M M 1 2 3 4 M M 1 2 3 4 M

pUC19 (High Copy) pET21b (Low Copy) pSTV28 (Low Copy)

M : 1kb Plus DNA Ladder 1% agarose Gel 5 ㎕ loading
1 : A사 (3 Step System) 2 : B사 (3 Step System) 3 : C사 (3 Step System) 4 : BIOFACT (2 Step System)