Page 181 - 2022-23 BIOFACT Catalogue

BIOFACT사
A사
M
M
C사
A사
BIOFACT사
B사
C사
B사
A사
BIOFACT사
1
M
2
1
M
3
3
2
pMW118
pET21b
Fosmid
M : 1Kb DNA ladder 1% agarose Gel 5 ㎕ loading
1 : Fosmid + insert 14kb Prep
2 : Fosmid
G : G사 Q : Q사 I : I사 B : BIOFACT
3 : Fosmid + insert 14kb DNA digestion
DNA / RNA Preparation
- Comparison of isolation yield on various plasmid copy numbers between HiGene™ Plasmid Mini Prep Kit (Ver.2.0),
2 Step system and other companies' prep kits (column type), 3 step system
M 1 2 3 4 M M 1 2 3 4 M M 1 2 3 4 M
pUC19 (High Copy) pET21b (Low Copy) pSTV28 (Low Copy)
M : 1kb Plus DNA Ladder 1% agarose Gel 5 ㎕ loading
M : 1kb DNA Ladder 1% agarose Gel 5 ㎕ loading
1 : A사 (3 Step System) 2 : B사 (3 Step System) 3 : C사 (3 Step System) 4 : BIOFACT (2 Step System)
1 : Company A (3 Step System) 2 : Company B (3 Step System) 3 : Company C (3 Step System) 4 : BIOFACT (2 Step System)
- Know for high yield
1. Cell culture time → At least 12 - 16 hrs
2. Culture volume → 3 ㎖ ~ 5 ㎖
3. Prepare 80% EtOH in a WB bottle and make fresh each time.
4. B2 Bu er mixed with RNase A (20 ㎎ / ㎖) should be stored at 4℃.
5. Please completely remove EtOH before using elution bu er.
6. Please use the kit prior to the expiration date.
7. Avoid vigorous vortexing during lysis step to prevent degradation of DNA.
8. Isolation e ciency can be increased when using EB bu er after pre-heating treatment for 10mins at 50℃ during DNA elution step.
(Especially, in case of long fragment)
9. In case it is di cult to separate the debris from supernatant, please perform again after incubation at 4℃ or incubation on ice.
10. When washing using NWB without ethanol, extraction time can be shortened by skipping column-idle step.
- Blue Indicator (Optional)
After B2 buffer
treatment
B1 buffer
Blue Indicator is a pH indicator which helps users visually check the pH changes during separa- B1 Bu er B2 Bu er 처리 후
tion process for plasmids. Users easily can use it after mixing B1 bu er before experiment. Blue 구성품목 (Contents)
Indicator in B1 bu er becomes transparent after B2 bu er step and it doesn't a ect the extraction B1 Spin Column & Collection Tube
e ciency of plasmid isolation. B2 RNase A
EB Blue Indicator
WB Bottle
Product Information
HiGene™ Plasmid Mini Prep Kit (Ver.2.0) [PM105-100]

▶Contents
B1 Spin Column & Collection Tube
B2 RNase A
EB Blue Indicator
NWB Help B
WB Bottle

Reference
1. CHOI, Yeon Hee, et al. A LuxR-type Transcriptional Regulator, PsyR, Coordinates Regulation of Pathogenesis-related Genes in Pseudomonas syringae pv. tabaci.
Journal of Life Science, 2015, 25.2: 136-150.
2. KIM, Dooil, et al. Escherichia coli ASKA Clone Library Harboring tRNA-Speci c Adenosine Deaminase (tadA) Reveals Resistance towards Xanthorrhizol.
Molecules, 2015, 20.9: 16290-16305.

Maker Cat.No. Product Size
PM105-100 HiGene™ Plasmid Mini Prep Kit (Ver. 2.0) 100 prep
BIOFACT
PM105-200 HiGene™ Plasmid Mini Prep Kit (Ver. 2.0) 200 prep
It is available to purchase individually in case of Spin Column & Collection Tube separately.

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BIOFACT CATALOGUE