Page 22 - 2022-23 BIOFACT Catalogue
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Isothermal Amplification
Description
Isothermal ampli cation method is a simple process that rapidly and e ciently accumulates nucleic acid sequences at constant tem-
perature, compared with PCR. LAMP (Loop-mediated isothermal ampli cation) is a technique for rapid and speci c ampli cation of
DNA based on a sophisticated primer design that allows the detection of a target gene within 30-60 minutes, at 65°C. BioFACT™ Bst
DNA Polymerase applicable to LAMP PCR is isolated from Bacillus stearothermophilus and it has strand-displacing 5′-3′ polymerase
activity but no 5' to 3' exonuclease activity.
Application
• Loop-mediated isothermal ampli cation(LAMP) • Whole genome ampli cation
• Isothermal ampli cation • RT-LAMP
Comparison of PCR & LAMP (Loop-mediated isothermal amplification) PCR
PCR LAMP PCR
Amplification Denaturation (95°C), primer annealing (-60°C) Isothermal ampli cation (60-72°C)
method & polymerization (72°C)
Instrument PCR thermal cycler Heating block, water bath & dry oven
Assay reaction time Over 90 mins. 30-60 mins.
Result Electrophoresis required (Conventional PCR) Naked eye detection available in real time
BIOFACT Product
BioFACT™ Bst DNA Polymerase (p. 108)
22 BIOFACT CATALOGUE
