Page 24 - 2022-23 BIOFACT Catalogue

UDG System Description

UDG System
PCR contamination remains an issue for laboratories performing detection of infectious agents and forensic procedures. The
single most important source of PCR product contamination is the generation of aerosols of PCR amplicons that is associated
with the post-PCR analysis. In addition to post-PCR contamination, the target template itself can be a source of contamination.
UDG System is mainly applied when performing PCR using a large number of samples such as PCR diagnosis or repeating the
same experiment. It is recommended to use in case reagents or equipment are contaminated by samples (product DNA) which
was used in the previous step.

1 Experiment 2 Experiment
st
nd
Sample A Sample B

C A G T C T C G T A
Sample B + UDG + dNTP(U) mix
G T C A G A G C A T
Contamination

dNTP(U) mix UDG
PCR (50℃, 3 min)
(dATP, dCTP, dGTP, dUTP) activation

C A G U C
G U C A G

PCR UDG inactivation
(PCR rst step)

1) PCR product from contaminated sample A 2) PCR product from sample B

U C G U A
C A G C A U U C G U A
A G C A U
U C G U A
C A G A G C A U
U C G U A
G C A G A G C A U U C G U A
U C G U A A G C A U
A G C A U
PCR product is degraded when U is cut Properly ampli ed DNA
Sample A Sample B

BIOFACT Product
Uracil DNA Glycosylase (UDG) (p. 112)

24 BIOFACT CATALOGUE