Page 57 - 2022-23 BIOFACT Catalogue

DNA / RNA Amplification Real-Time PCR Enzyme

SFCgreen® I

Outstanding Intercalating Dye 100% Made in Korea

SFCgreen® I is a green uorescent dye with excitation and emission wavelengths very similar to those of SYBR® green I or Eva-
Green™. The DNA binding mechanism is a bis-intercalator that binds to the DNA minor groove and emits light.
SFCgreen® I has high DNA binding strength and shows a higher sensitivity compared to SYBR® green I or EvaGreen™. Because
PCR inhibition is low, PCR ampli cation e ciency is high even at high concentrations, and it is stable under light, heat, and
chemicals, resulting in a longer uorescence half-life compared to other uorescent dyes.

Feature
• λ abs / λ em = 459/522 nm (DNA bound) • Superior bright fluorescence & Long half-life
• Similar wavelength with that for SYBR®green I or EvaGreen™ • HRM / Melting curve analysis available
• Less PCR inhibition by dye concentration • Non-mutagenic, Non-cytotoxic

SFCgreen® I Wavelengths for SFCgreen® I, SYBR®Green I & EvaGreen™
100
90 excitation emission
fluorescence excitation or emission(%) 60 50 40 30
Minor groove binder Bis-intercalator 80 70

10 20
Major groove Minor groove 0
300 350 400 450 500 550 600 650 700
Wavelength (nm)
Dye Excitation Emission
SFCgreen® I 459 nm 522 nm
EvaGreen™ 500 nm 530 nm
SYBR®Green I 497 nm 520 nm

QC DATA
Comparison of thermal stability between SFCgreen® I and EvaGreen™
Melt Curve
[Idle time test]
0.059 Melt Curve
0.100 0.059
0.100 EvaGreen TM SFCgreen® I
EvaGreen TM SFCgreen® I
0.049
0.080 SFCgreen® I 0.049
0.080 SFCgreen® I 0.039
0.060 0.039
0.060 Derivative Reporter (-Rn) Derivative Reporter (-Rn)
0.040 0.029 0.029
0.040
0.019
0.019
0.020
0.020
0.0090 0.0090
0.000
0.000
0 hr
0 hr 0.5 hr 1 hr 1 hr 2 hr 2 hr 3 hr 3 hr 65.0 70.0 75.0 80.0 85.0 90.0 95.0
0.5 hr
65.0 70.0 75.0 80.0 85.0 90.0 95.0
Temperature (℃) Temperature (℃)
: 95℃ / 0 hr : 95℃ / 0.5 hr : 95℃ / 1 hr : 95℃ / 2 hr : 95℃ / 3 hr
* SFCgreen® I dye has better thermostability than EvaGreen™.
(Small deviation in absorbance compared to EvaGreen™)
57
BIOFACT CATALOGUE