Page 55 - 2022-23 BIOFACT Catalogue

DNA / RNA Amplification Real-Time PCR Enzyme

What is Passive Reference

Dye? (e.g. ROX,Fluorescein dye etc.)

Passive reference dye is used as a reference point to correct the uorescence signal in real-time PCR or to calculate the uctuation
of the uorescence signal. Calibration means modifying to minimize the di erence between the total volume or the equipment
scanner during the reaction. In general, most real-time PCR equipment uses ROX dye as a passive reference dye. This is because
ROX dye does not have any e ect on real-time PCR reaction and emits a uorescence signal that is clearly distinguished from probe
uorescent dye (reporter or quencher). As with some equipment from Bio-Rad, there are some that use uorescein dye and there
are systems that do not require a passive dye such as the Rotor-gene. It is recommended to select the passive reference dye to be
used after considering the advantages and disadvantages of each device or method.

Required , Optional

Agilent (Stratagene) Mx3000P, Mx3005P, Mx4000, AriaMx
Analytik Jena qTOWER
Bioer LineGene, LineGene K, LineGene 9600, LineGene 9640, LineGene 9660
Bioneer Exicycler96
Bio-Rad CFX96, CFX384, Chromo4, MiniOpticon, Opticon, Opticon2

BJS Xxpress
Cepheid SmartCycler, SmartCycler2
Eppendorf Mastercycler ep realplex, Mastercycler ep realplex-2S
Low-ROX Fluidigm BioMark
Hain Lifescience FluoroCycler 96
Illumina Eco
Life Technologies 7500, 7500 FAST, QuantStudio 12k Flex, ViiA7

Qiagen Rotor-Gene 3000, Rotor-Gene 6000, Rotor-Gene Q
Roche LightCycler 1, LightCycler 2, LightCycler 96, LightCycler 480, LightCycler nano
Takara Thermal Cycler Dice (TP800)
Techne PrimeQ, Quantica
Thermo Piko Real

Life Technologies PRISM 5700, 7000, 7300, 7700, 7900, 7900HIT, 7900HIT FAST
High-ROX
StepOne, StepOnePlus

Fluorescein Bio-Rad iCycler iQ, MyiQ, MyiQ2, MyiQ5

55
BIOFACT CATALOGUE