Page 72 - 2022-23 BIOFACT Catalogue

BioFACT™ OneStep Multi-Star

qRT-PCR Series ⓗ (For Probe)

One-step RT-qPCR using RNA as a Template

BioFACT™ OneStep Multi-Star qRT-PCR Kit (For Probe) is designed to perform one-step RT-qPCR in a single tube combining
BioFACT™ RTase and BioFACT™ 2X Multi-Star Real-Time PCR Master Mix (for Probe). It is optimized PCR mixture for probe-based
Real-Time PCR platforms using sequence-speci c probe method and it is available for use on any Real-Time PCR instruments.

Feature
• High sensitivity • Use of any sequence-speci c probe
• Easy sample optimization • OneStep qRT-PCR
• Low copy transcripts ampli cation

Application
• Quanti cation of target RNA using RT-PCR • Various applications using qPCR

QC DATA

- Comparison of sensitivity in various concentrations of template DNA between BioFACT™ OneStep Multi-star qRT PCR Kit
(For probe) and the other company's Onestep RT-qPCR Kit (Limit of Detection)

[GAPDH]

BIOFACT Company A [GAPDH] Target : Human total RNA
△Ct Value 100 ng 20 ng 4 ng 0.8 ng
BIOFACT 18.40 21.43 25.70 29.38
Company A 19.66 23.27 26.42 30.39

[MAPK1]

[MAPK1] Target : Human total RNA
BIOFACT Company A
△Ct Value 100 ng 20 ng 4 ng 0.8 ng
BIOFACT 20.90 23.61 26.09 29.54
Company A 21.35 24.31 26.84 30.87

72 BIOFACT CATALOGUE