Page 70 - 2022-23 BIOFACT Catalogue

BioFACT™ Multi-Star Real-Time

PCR Master Mix ⓗ (For Probe)

Probe-based Real-Time PCR Master Mix used in Development of Various Molecular Diagnostics Kits

BioFACT™ 2X Multi-Star Real-Time PCR Master Mix (For Probe) is optimized PCR mixture for probe-based Real-Time PCR platforms
using sequence-speci c uorogenic probe chemistries.

Application
• Quanti cation of target DNA using Real-Time PCR
• Quanti cation of target RNA using RT-PCR
• Various applications using qPCR ; Genotyping, Diagnosis

QC DATA

- Example of usage in various genotyping applications

[FAM] [VIC] [Cy5]

Target : Human genomic DNA
Gene : MAPK1(FAM), GAPDH(VIC), β-2M(Cy5)

- Comparison of activity in various concentrations of template between BioFACT™ 2X Multi-Star Real-Time PCR Master Mix
(For Probe) and other Real-Time PCR Master mixes from other companies

BIOFACT Company A

△Ct Value 50 ng 10 ng 2 ng 0.4 ng
BIOFACT 22.35 24.78 27.00 29.51
Company B Company C
Company A 23.36 25.84 27.59 30.08
Company B 24.65 26.95 29.18 31.62
Company C 22.73 25.23 27.48 29.84
Target : Human genomic DNA
Gene : MAPK1(FAM)

70 BIOFACT CATALOGUE