Page 69 - 2022-23 BIOFACT Catalogue

DNA / RNA Amplification Real-Time PCR Enzyme

FastFACT™ Real-Time PCR

Master Mix (For Intercalating Dye)

3X Faster than Standard Real-Time PCR Kit

FastFACT™ 2X Real-Time PCR Master Mix (For Intercalating Dye) uses the bu er and enzyme optimized for fast Real-Time PCR to
signi cantly reduce the time required for each step of PCR (denaturation, annealing, extension), from at least 2 hours to within 45
minutes.

Application
• Fast qPCR Mixture (Reaction time: within 45 mins.) • Antibody-mediated Hot Start PCR enzyme
• Low-copy transcripts amplification

QC DATA
- Comparison of activity by various template concentrations
(Comparison of amplification patterns between standard qPCR Mix and FastFACT™ Real-Time PCR Master Mix by reducing the time of each PCR step to 1 sec.)

Target : Human genomic DNA Target : Plant genomic DNA
Gene : GAPDH Gene : GAPDH

△Ct Value 50 ng 10 ng 2 ng 0.4 ng △Ct Value 50 ng 10 ng 2 ng 0.4 ng
BIOFACT Standard Type N/A 44.63 N/A 44.66 BIOFACT Standard Type N/A N/A N/A N/A
BIOFACT [FDQ388] 19.91 21.72 23.94 26.43 BIOFACT [FDQ388] 16.58 19.62 23.25 27.1
BIOFACT Standard Real-Time PCR Master Mix BIOFACT Standard Real-Time PCR Master Mix
BIOFACT Fast Real time PCR Master Mix[FDQ388]
BIOFACT Fast Real time PCR Master Mix[FDQ388]

Product Information
Master Mix Type (For Intercalating Dye)
FastFACT™ 2X Real-Time PCR Master Mix including SYBR® Green I [FDQ387-40h]

▶Contents
FastFACT™ 2X Real-Time PCR
Master Mix (including SYBR® Green I) [ 1 ㎖ x 4 ea]
▶Expiration date
1 Year at -20 ± 5℃

Type Cat.No. Product Size
FDQ387-40h FastFACT™ 2X Real-Time PCR Master Mix including SYBR® Green I in mixture, w/o Rox reference dye 1 ㎖ × 4 ea
Master Mix FDQ388-40h FastFACT™ 2X Real-Time PCR Master Mix including SYBR® Green I in mixture, with low Rox reference dye 1 ㎖ × 4 ea
FDQ389-40h FastFACT™ 2X Real-Time PCR Master Mix including SYBR® Green I in mixture, with high Rox reference dye 1 ㎖ × 4 ea

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BIOFACT CATALOGUE