Page 67 - 2022-23 BIOFACT Catalogue

DNA / RNA Amplification Real-Time PCR Enzyme

BioFACT™ OneStep qRT-PCR

Master Mix (For Intercalating Dye)

One-step RT-qPCR using RNA as a Template

BioFACT™ 2X Multi-Star OneStep qRT-PCR Master Mix (For Intercalating dye) is an optimized product which allows the qRT-PCR
to be done in one PCR tube by creating an enzyme mixture with Thermostable BioFACT™ RTase and H-Star(A-Star) Taq DNA poly-
merase, which has Hot Start PCR features. Intercalating dye can be selected from either SFCgreen® I or SYBR® Green I, and it can be
conveniently used simply by adding the primer and the template in order to perform PCR. This product can be used with all types
of Real-Time PCR instruments.

Feature
• High sensitivity using BioFACT™ H-Star(A-Star) Taq • Enzyme mixture of BioFACT™ RTase and
DNA polymerase H-Star(A-Star) Taq DNA Polymerase)
• Pre-denaturation time : 15 mins. • Intercalating dye : SYBR® Green I & SFCgreen® I

Application
• Quanti cation of target RNA using RT-PCR • Various applications using qPCR

QC DATA
- Comparison of sensitivity by product type (Limit of Detection)

RQ321-10h Target : Human total RNA RQ321-10h Target : Human total RNA
RQ351-10h Gene : α-CateninD RQ361-10h Gene : GAPDH
△Ct Value 50 ng 10 ng 2 ng 0.4 ng △Ct Value 50 ng 10 ng 2 ng 0.4 ng
RQ321-10h 20.29 20.10 22.5 22.48 24.87 24.72 27.44 27.42 RQ321-10h 17.56 17.59 20.05 19.84 22.31 22.18 24.68 24.61
RQ351-10h 20.25 20.26 22.43 22.49 24.7 24.89 27.27 27.27 RQ361-10h 17.46 17.56 19.84 19.86 22.3 22.25 24.81 24.68
* Comparison of sensitivity between RQ321-10h and RQ351-10h * Comparison of sensitivity between RQ321-10h and RQ361-10h
(Similar Ct values were shown when SYBR® Green I Dye was added to RQ321-10h for comparison test.) (Similar Ct values were shown when SYBR® Green I Dye was added to RQ321-10h for comparison test.)

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BIOFACT CATALOGUE