Page 84 - 2022-23 BIOFACT Catalogue

BioFACT™

H-Star Taq DNA Polymerase

Highly Speci c Ampli cation of Less Than 1 kb Size Target DNA

BioFACT™ H-Star Taq DNA Polymerase is a chemical-mediated Hot Start Taq DNA polymerase which leads to complete inactivation of
the polymerase until the initial heat activation step. Through this feature, it provides high PCR speci city, avoiding extension of non
speci c annealed primers or primer-dimers and it is highly suitable for e cient ampli cation of di cult templates caused by high GC
content or complex structure. BioFACT™ H-Star Taq DNA Polymerase is optimized to amplify less than 1 kb size target DNA and it can
be supplemented by increasing the cycle as ampli cation e ciency and productivity of chemical-mediated Hot Start PCR enzymes
may be lower than general Taq DNA Polymerase.
Feature
• Source : Thermus aquaticus • Hot Start activity (Chemical-mediated Hot Start)
• 5' → 3' exonuclease activity : Yes • A-tailing : Yes
• 3' → 5' exonuclease activity ( delity) : No • Error rate : 12 - 13 bp error / 10 bp
6
• Ampli cation size : < 1 kb PCR

Application
• Hot Start PCR • TA cloning • High speci city PCR
• Real-Time PCR • Multiplex PCR • Allele speci c PCR
• High G+C rich / Repeat region PCR • Genotyping • Suitable for UDG System

QC DATA
- Comparison of Hot Start PCR enzyme activity between - Comparison of amplification efficiency according to
BioFACT™ H-Star Taq DNA Polymerase and other various template concentrations
companies' Hot Start PCR enzymes

ApoE (0.9 kb) APEX (1 kb) ApoE (0.9 kb) APEX (1 kb)
M A B C D E F M M A B C D E F M M A B C D E F M M A B C D E F M
BIOFACT BIOFACT

Company A Company A
Company B Company B

Company C Company C
Target : Human genomic DNA 10 ng PCR Condition : 60°C, 35 cycles Target : Human genomic DNA PCR Condition : 60°C, 35 cycles
M : 1kb DNA Ladder M : 1kb DNA Ladder
A : 2.5 U B : 1.25 U C : 0.625 U D : 0.313 U E : 0.156 U F : 0.078 U A : 50 ng B : 10 ng C : 2 ng D : 0.4 ng E : 0.08 ng F : 0.016 ng

- Amplification data of various target genes using H-Star Taq at fixed annealing temperature

Various DNA detection at fixed annealing temperature (55℃)
PCR was carried out at the same annealing temperature condition using human
genomic DNA 100 ng/㎕ and 80 kinds of primer sets.

84 BIOFACT CATALOGUE