Page 109 - Abstract book - TJSSF-2020

Thailand – Japan Student Science Fair 2020 (TJ-SSF 2020)
“Seeding Innovations through Fostering Thailand – Japan Youth Friendship”

The Portable Dark Field Microscope

Panuphong Northao , Natwara Thongsak
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Advisors: Wipawee Srihanon , Sophon Klomkliang , Atcha Kopwitthaya
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Princess Chulabhorn Science High School Lopburi
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National Electronics and Computer Technology Center
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Abstract

The portable dark field microscope will be available at the school for using with dark field microscopy
and will have 25x, 50x, 100x magnifications for appropriately sized specimens. The principle of light
scattering applied in dark field microscopy. The dark field microscopy shows the specimens bright on
a dark background and they appear bright because they scatter the light from the light source into the
sample. The portable dark field microscope consists from 3 parts which are an objective lens, slide
holder with light source and focus controller which works together. The LED holder and the focus
controller were designed by SketchUp software then printed with PLA filaments using 3D printer. For
other parts, we cut acrylic sheets by laser cutting machine and gather all the pieces to become a device.
In addition, there is a special light scattering condition that will have the color of the image on the
opposite side of the color wheel, for example, an image of an early embryo of frog, which was pink-red
in a portable bright field microscope, but green in a portable dark field microscope. Although, gold
nanoparticles have surface plasmon resonance properties which go with the principle of light scattering.
So, we use gold nanoparticles to check our resolution as the same as the live sample and dry sample.

Keywords: dark field microscopy, gold nanoparticles, surface plasmon resonance,
bright field microscopy

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