Page 125 - Clinical Immunology_ Principles and Practice ( PDFDrive )

108 ParT ONE Principles of Immune Response

Fetal liver Periphery
Bone marrow
Antigen Subject to Antigen
independent antigen selection dependent
MψLC M M M
TdT µ µ µ D
Ig

Stem ProB Early Late Immature Mature Activated Plasma
cell cell preB cell preB cell B cell B cell B cell cell
TdT
VpreB, λ14.1
Ig α, β
DQ52→J(fetal)
D→J
V→DJ
V→J
slg
CD34
CD10
CD19
CD20
CD21
CD24
CD38
FIG 7.1 Model of B-Cell Differentiation. B-cell development is typically viewed as a linear progres-
sion through different stages of differentiation. The various processes associated with the assembly
of the B-cell receptor (BCR) complex and the expression pattern of surface molecules whose
presence or absence are illustrated through use of bars. The various steps in immunoglobulin
(Ig) rearrangement and the pattern of expression of these surface molecules can be used to
characterize stages in B-cell development.

M
D
G, A, E
M M M M M
D D D D

Memory
Memory
Immature Transitional Naive mature Unswitched switched Plasmablast
T1 T2 T3 (MZ-like)
IgM ++ +++ ++ + + + - -
IgD - + ++ ++ ++ + - -
R123 + + + Int - + + +
CD10 + + +/- -/+ - - - +/-
CD19 + + + + + + + lo
CD24 +++ +++ ++ + + + - -
CD27 - - - - - + + hi
CD38 +++ +++ ++ + + lo lo hi
FIG 7.2 B-Cell Subsets in Peripheral Blood. Peripheral blood B-cell subsets can be identified
by differential staining for immunoglobulin M (IgM), IgD, CD10, CD19, CD24, CD27, and CD38
and by the use of the rhodamine dye R123, which is extruded by the ABCB1 transporter expressed
in naïve, mature B cells and, to a lesser extent, T3 transitional B cells. B cells that are CD20 +
36
4
+
−
CD27 CD43 CD70 are currently the best candidates for the human B-1-cell counterparts, but
+
this is subject to change. In addition to these subsets found in blood, germinal center B cells
−
+/−
found in the spleen, and lymph nodes are characterized as IgD CD38 CD10 CD27 ; marginal
++
+
zone (MZ) B cells are typically CD27 CD21 CD23 CD1c IgD ; and long-lived plasma cells found
++
+
+/−
−
+
−
+
in bone marrow, the spleen, and tonsils are CD138 CD20 CD38 .
++