Page 1321 - Clinical Immunology_ Principles and Practice ( PDFDrive )

CHaPtEr 94 Assessment of Neutrophil Function 1282.e1

MU lt IP l E-CHOICE QUES t IO n S

1. Results from laboratory testing show that neutrophils from of fluorescence with a moderate shift to the right that did
a patient exhibit a negative dihydrorhodamine (DHR) analysis not fall within the normal range. What is the MOST likely
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but exhibit normal/supranormal production of O 2 by interpretation of this finding?
cytochrome c reduction. What is the MOST likely reason(s) A. The subject most likely has CGD with a defect in p47 phox .
for these results? B. The subject is most likely a carrier of gp91 phox deficient
A. The patient probably has myeloperoxidase (MPO) defi- CGD.
ciency; the discrepancy in these test results should be C. The subject most likely has CGD with a defect in gp91 phox .
resolved by Kaplow peroxidase staining of the polymor- D. The subject is most likely normal.
phonuclear neutrophil (PMN) preparation and/or MPO 4. When performing Western blot analyses for the detection of
enzyme-linked immunosorbent assay (ELISA). components of nicotinamide adenine dinucleotide phosphate
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B. The patient has no immune defect since the O 2 -dependent (NADPH) oxidase, what is the MOST important reason to
ferricytochrome c assay falls with the normal range. run a control from a patient with a known mutation in
C. The patient has chronic granulomatous disease (CGD). gp91 phox ?
D. None of the above.
A. It is performed to serve as validation of the assay.
2. Why are neutrophils treated with the fluorescent dye, calcein- B. It is performed to ensure adequate development of p22 phox
AM, in the adherence assay? for the correct identification of the impaired protein of
A. Calcein is needed to activate the cells. the membrane component.
B. Calcein is needed to activate the integrins. C. It is only important to run a control from a normal subject
C. The calcein fluorescence of the adherent cells can be for band identification and intensity comparisons
quantified with the use of a fluorescent plate reader. D. Only molecular weight markers are needed to run to serve
D. None of the above. as band identification.
3. The DHR analysis of neutrophils stimulated with phorbol
myristate acetate (PMA) from a patient yielded a broad peak