Page 634 - Clinical Hematology_ Theory _ Procedures ( PDFDrive )

30 Principles of Blood Collection and

CHAPTER Instrumentation in Hematology*
Processing

KEY TERMS

bim odal cellular distribution Gaussian distribution photon

Delta checks histogram red cell distribution width (RDW)
digital cell m orphology LASER sam ple

electrical im pedance monoclonal antibodies statistic
ow-cell mean peroxidase index Stokes Shift

ow cell cytom etry mean platelet volum e (MPV) VCS (volum e, conductivity, scatter)
uorochrom e MPV nom ogram viscosity

uorophore param eter

LEARNING OUTCOMES

Instrumental principles ■ Describe the relationship of the RDW to the mean corpuscular vol-

■ Describe the basic theory of the electrical impedance principle of ume (MCV).
cell counting and sizing. ■ Name the six classi cations of erythrocytes based on the RDW and

.
■ Describe the basic theory of the optical detection principle of cell MCV
counting and sizing. ■ Explain how the red cell mean index (RCMI) is calculated and give

■ Explain the fundamental concepts of laser technology. the normal value.

■ Describe the principles of ow-cell cytometry and two basic uses of ■ Describe the appearance of a leukocyte histogram generated by the
this technology in hematology. electrical impedance method.

■ Describe the appearance of a leukocyte histogram generated by the
Whole blood cell analysis optical detection method.

■ De ne the terms parameter and sample. ■ Describe the construction of a platelet histogram.

■ List the parameters measured by basic benchtop hematology ■ Explain how the mean platelet volume (MPV) is calculated.

analyzers. ■ Compare the relationship between MPV and the platelet count.

■ Describe the methods used to measure the parameters named in ■ Name at least four disorders in which the MPV is abnormal.

the preceding objective. ■ Explain the purpose of the platelet distribution width (PDW) and its

■ Name the parameters measured by total cell counting systems. normal value.

■ De ne the abbreviation RDW.

■ Describe the process and output of total cell and histogram electri- Laser technology

cal impedance systems. ■ Describe the generation, by laser technology, of a histogram for red

■ Describe the process and output of a laser scatter technology blood cells (RBCs).

system. ■ Explain how a platelet histogram is generated.
Compare the process and output of the continuous ow system to ■ Describe the analysis and interpretation of the peroxidase analysis.

the other two types of total cell and differential cell counters. ■ Explain the output of the basophil/lobularity channel.

■ Describe the general characteristics of histograms. ■ Describe the process of lymphocyte subtyping.

Analysis of instrumental data output Applications of ow cytometry

■ Describe the appearance of microcytic and macrocytic erythrocytes ■ Describe the general functions that ow cytometry analysis can

on a histogram. provide.

■ Name two conditions that would contribute to a bimodal cellular ■ Name the three factors that have contributed to the rapid advance

distribution on an erythrocyte histogram. of the technology of ow cytometry.

■ Explain how the red cell distribution width (RDW) is calculated and ■ Name and discuss three hematological applications of ow cytometry.

give the normal range. ■ Name and discuss three other cellular applications of ow cytometry.
*

*Additional procedures, in CLSI format, are provided on this book’s companion Web site at thepoint.lww.com/Turgeon6e.

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