Page 686 - Clinical Hematology_ Theory _ Procedures ( PDFDrive )

Principles of Blood Collection and ures
32 Laboratory Manual: Manual Proced

CHAPTER in Hematology
Processing

LEARNING OUTCOMES

■ Describe the general principles of basic and selected special- ■ Perform the stated procedure.
ized procedures in hematology, special stains, and coagulation ■ Perform any calculations needed for reporting the results in the

procedures. procedure.

■ Describe the proper type of specimen collection and handling for ■ State the reference range values for the parameters measured by
the stated procedure. the procedure.

■ Prepare the necessary reagents for the stated procedure. ■ Describe the sources of error and clinical applications of the

■ Describe the quality control steps needed for the stated procedure. procedure.

PROCEDURAL FORMAT 9. Reporting results (normal values)
10. Proce ure notes inclu ing sources o error, clinical

T e proce ures in this chapter are presente in a ormat that is applications, an limitations o the proce ure

consistent with the gui elines set orth by the CLSI. T is ormat is: 11. Re erences

1. Proce ure title an specif c metho All specimens shoul be treate with caution. All bloo ,

2. est principle inclu ing type o reaction an the clinical tissues, an bloo erivatives shoul be consi ere poten-

reasons or the test tially in ectious. Specimen han ling notes that are particularly

3. Specimen collection an preparation important to coagulation stu ies are presente on the page

4. Reagents, supplies, an equipment imme iately prece ing the Coagulation Proce ures section.

5. Calibration o a stan ar curve Many o the proce ures in this chapter are classic metho s that

6. Quality control are in requently per orme in the working clinical laboratory.

7. Proce ure Tese proce ures are inclu e or use in special circumstances

8. Calculations such as in the stu ent laboratory or small clinical laboratories.

HEMATOLOGY PROCEDURES

TOTAL LEUKOCYTE COUNT PROCEDURE Reagents, Supplies, and Equipment

Principle 1. Disposable Leuko- IC capillary pipette an Leuko- IC

A capillary bloo specimen or an ED A-anticoagulate whole iluent vial

bloo specimen is collecte in a 20-µL capillary pipette mixe 2. Han hel cell counter (optional)

with a solution o acetate bu er an gentian violet stain. T e 3. Conventional microscope an lens paper

erythrocytes will lyse, an the stain will impart a light violet- 4. ra itional hemocytometer an coverslip or isposable

blue color to leukocytes to acilitate enumeration. Although total hemocytometer.*

WBC counts are most requently per orme with automate Note: T e improve Neubauer hemocytometer consists

equipment, the manual enumeration o WBCs is important in o two raise counting chambers. A specif cally weighe

cases where the counts are very low.

Specimen
*A isposable, glass hemocytometer (www.bioanalytic. e) has a gri
Anticoagulate whole bloo or capillary bloo can be use . pattern an epth that are the same as the tra itional Neubauer hemo-
ED A is the pre erre anticoagulant. A hemolyze specimen cytometer. T is all-in-one unit oes not require a coverslip. Follow the

is inappropriate or an accurate erythrocyte count. manu acturer’s instructions or use.

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