Page 684 - Clinical Hematology_ Theory _ Procedures ( PDFDrive )

668 PART 8 ■ Fundamentals of Hematological Analysis

REVIEW QUESTIONS (continued)

3. PCR testing is use ul in 9. All o the ollowing are true o FISH except

A. orensic testing A. he acronym stands or luorescent in situ

B. genetic testing hybridization.

C. disease diagnosis B. It is a tissue-based molecular diagnostic assay.

D. All o the above C. It is a prenatal diagnosis o a genetic disorder.

D. It is use ul in the diagnosis o various anemias.

4. T e traditional PCR technique

A. extends the length o the genomic DNA *10. Microarrays are

B. alters the original DNA nucleotide sequence A. DNA probes bonded on glass chips

C. ampli es low levels o speci c DNA sequences B. tissue-based probes

D. ampli es the target region o RNA C. used to identi y single-base mutations

D. used to determine clonality in lymphomas

5. PCR protocol

A. doubles the speci c amount o DNA with each cycle *11. Molecular techniques provide a diagnostic tool to

B. typically has three temperature steps A. detect MRD in hematological malignancies

C. repeats the number o cycles about 30 B. monitor patients ollowing bone marrow

D. all o the above transplantation

C. detect an early relapse in a patient treated or a

6. Variations o PCR include hematological malignancy

A. nested primers D. all o the above

B. real-time PCR

C. microarray analysis *12. Which o the ollowing best describes the molecular

D. both A and B alteration o the Philadelphia chromosome to chronic

myelogenous leukemia (CML)?

7. T e method that continues to be considered the “gold A. Te mutation produces an overly active tyrosine

standard” or DNA sequencing kinase that increases apoptosis.

A. Sanger method B. Te mutation reduces serine enzyme activity in

B. Southern blot method a ected cells.

C. Northern blot method C. T e mutation involves translocation o a gene that is

D. Dot blot method critical to myelocytic maturation.

D. Hematopoietic cells gain a proli eration advantage

8. Te Southern blot procedure has diagnostic applications because o tyrosine kinase activity.

or diseases or disorders associated with

A. signi cant changes in DNA (e.g., deletion)

B. determination o clonality in lymphomas o - or

B-cell origin

C. detection o restriction ragment length polymorphisms

D. all o the above

COMPANION RES OURCES BIBLIOGRAPHY

http://thepoint.lww.com/ urgeon6e Bao YP, et al. SN identi cation in unampli ed human genomic DNA

with gold nanoparticle probes, Nucleic Acids Res, 33(2):1–7, 2005.
Each student is encouraged to access and use the Web- Béné MC, Kaeda JS. How and why minimal residual disease studies

based companion resources developed or this chapter. are necessary in leukemia, Haematologica, 94(8):1135–1150, 2009.

Here, you will nd additional learning tools to increase Bruns DE, Ashwood ER, Burtis CA. Fundamentals o Molecular

your understanding o the concepts and clinical applica- Diagnostics, St. Louis, MO: Elsevier, 2007.

tions o the chapter. Croce CM. Oncogenes and cancers, N Engl J Med, 358(5):502–510,
2008.