Page 177 - 2022-23 BIOFACT Catalogue

Column Type
Solution Type
Magnetic Bead Type DNA / RNA Preparation
Easy Type

Magnetic DaBead™ Easy
Solution
Column
Bead Type Type Type Type
Plasmid Prep Kit

High-E ciency Plasmid Extraction using Magnetic Bead

DaBead™ Plasmid Prep Kit is a kit for extracting Plasmid DNA using Magnetic Bead. DaBead™ Magnetic bead is designed for
nucleic acid puri cation by coating the surface of Super Paramagnetic Nanoparticle with silica. As the extraction process
are similar to the column typed method, it is easy to use even without prior experience, and it can be used conveniently by
minimizing the centrifugation step. DaBead™ Plasmid Prep Kit is designed for plasmid DNA extraction in any scale (mini,
midi and maxi prep) using one stand (DaBead™ Magnetic Separation Stand).
Feature
• Magnetic Bead type • Easy to use even without prior experience with simple puri cation steps
• Various scaled extraction (Mini, midi & maxi prep) • Cell debris removal filter included (Optional)
Specifications
Mini scale Midi scale Maxi scale
High Copy 1 ~ 3 ㎖ 20 ~ 50 ㎖ 50 ~ 100 ㎖
Culture Volume
Low Copy 3 ~ 5 ㎖ 50 ~ 100 ㎖ 100 ~ 200 ㎖
Typical Yield < 20㎍ > 200㎍ > 500㎍

Workflow

- DaBead™ Plasmid Prep Kit _ 1.5 ㎖ Magnetic Stand [PM751-100]
DNA Binding Transfer
< PM751> Magnetic Bead eluted DNA

Transfer of
supernatant after
Cfg.
Lysis & protein precipitation
Cell harvest DNA binding & washing DNA elution
& remove cell debris
< PM753 - Cell Debris Removal Syringe Filter Type >
DNA Binding Transfer
Magnetic bead eluted DNA
Cell debris ml 20 ml 20
removal 15 15
syringe filter 10 5 10 5

Lysis & protein precipitation
Cell harvest DNA binding & washing DNA elution
& remove cell debris

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BIOFACT CATALOGUE

ml 20 15 10 5 ml 20 15 10 5