Page 175 - 2022-23 BIOFACT Catalogue
P. 175
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1
2
M
M
E.coli
HEK293F Cells
Perilla Leaf (깻잎)
(Wax-covered leaf)
(Animal tissue)
(Animal Cultured cell)
M : 1kb DNA Ladder
M : 1kb DNA Ladder
M : 1kb DNA Ladder
M : 1kb DNA Ladder
M : 1kb DNA Ladder
1 : A사
1 : A사
1 : A사
1 : A사
1 : A사
2 : B사
2 : B사
2 : BIOFACT
2 : B사
2 : B사
3 : BIOFACT
3 : BIOFACT
3
3 : BIOFACT
3 : BIOFACT
M
3
M
M
M
M 1 1 2 2 3 3 M 1 1 2 2 M 1 1 2 2 3 3 M M M 1 1 1 2 2 2 3 3 3 Persimmon Leaf (감잎) M 1 1 2 2 Bovine Liver (소간) 3 M 1 2 3 M 1 2 3
M 1 2 3 M 1 2 3 M 1 2 3 M 1 2 3 M 1 2 3
Perilla Leaf (깻잎) Persimmon Leaf (감잎) Bovine Liver (소간) HEK293F Cells E.coli
M : 1kb DNA Ladder (Wax-covered leaf) (Animal tissue) (Animal Cultured cell) M : 1kb DNA Ladder
DNA / RNA Preparation
M : 1kb DNA Ladder M : 1kb DNA Ladder M : 1kb DNA Ladder
1 : A사 1 : A사
1 : A사 1 : A사 1 : A사
2 : B사 2 : B사
Perilla frutescens (Leaf) Kaki persimmon Bovine liver HEK293F Cells E.coli
2 : BIOFACT
3 : BIOFACT 2 : B사 2 : B사 3 : BIOFACT
M : 1kb DNA Ladder (Leaf, Wax-coated) (Animal tissue) (Animal Cultured cell) M : 1kb DNA Ladder
3 : BIOFACT
3 : BIOFACT
1 : Company A M : 1kb DNA Ladder M : 1kb DNA Ladder M : 1kb DNA Ladder 1 : Company A
Perilla Leaf (깻잎) Persimmon Leaf (감잎) Bovine Liver (소간) Bacillus sp. E.coli
1 : Company A
2 : Company B 1 : Company A 1 : Company A M : 1kb DNA Ladder (Wax-covered leaf) 2 : Company B (Animal tissue) M : 1kb DNA Ladder M : 1kb DNA Ladder
2 : Company B
3 : BIOFACT 2 : BIOFACT 2 : Company B 1 : 10 mg M : 1kb DNA Ladder 3 : BIOFACT M : 1kb DNA Ladder 1 : 1X10 7 1 : 1X10 7
3 : BIOFACT 3 : BIOFACT 1 : 10 mg 1 : 10 mg
2 : 50 mg
- Comparison of total RNA isolation efficiency on various sample scales 2 : 50 mg 2 : 20 mg 2 : 1X10 8 2 : 1X10 8
3 : 1X10 9
3 : 100 mg
3 : 1X10 9
2
M 1 2 3 M 1 2 3 M 1 2 3 M 1 3 : 100 mg 3 M 1 2 3 3 : 30 mg
<Plant> <Animal Tissue> <Bacterium - Gram+ >
M 1 2 3 M 1 2 3 M 1 2 3 M 1 2 3 M 1 2 3 * 상기 결과는 모두 DNase I 처리 전 입니다.
Perilla Leaf (깻잎) Persimmon Leaf (감잎) Bovine Liver (소간) Bacillus sp. E.coli
M : 1kb DNA Ladder (Wax-covered leaf) (Animal tissue) M : 1kb DNA Ladder M : 1kb DNA Ladder
1 : 10 mg M : 1kb DNA Ladder M : 1kb DNA Ladder 1 : 1X10 7 1 : 1X10 7
2 : 50 mg 1 : 10 mg 1 : 10 mg 2 : 1X10 8 2 : 1X10 8
Perilla frutescens (Leaf) Kaki persimmon Bovine Liver Bacillus sp. E.coli
2 : 20 mg
2 : 50 mg
3 : 100 mg 3 : 1X10 9 3 : 1X10 9
M : 1kb DNA Ladder (Leaf, Wax-coated) (Animal tissue) M : 1kb DNA Ladder M : 1kb DNA Ladder
3 : 30 mg
3 : 100 mg
1 : 10 mg M : 1kb DNA Ladder M : 1kb DNA Ladder 1 : 1X10 7 1 : 1X10 7
2 : 50 mg 1 : 10 mg 1 : 10 mg 2 : 1X10 8 2 : 1X10 8
3 : 100 mg 2 : 50 mg 2 : 20 mg 3 : 1X10 9 10 ng 3 : 1X10 9
3 : 100 mg 3 : 30 mg * 상기 결과는 모두 DNase I 처리 전 입니다.
0.4 ng
2 ng
* Before DNase I treatment
0.08 ng
* Before DNase I treatment
Gene : GAPDH Plot : RNA 10 ng, 2 ng, 0.4 ng, 0.08 ng
- Amplification efficiency of RNA using HiGene™ Total RNA Prep Kit (qRT-PCR)
Product Size : 249 bp ABI 7500 FAST
10 ng 10 ng
0.4 ng
2 ng 0.4 ng 2 ng
[nt] [nt] 0.08 ng
10 ng 0.08 ng Plant tissue (Leek) Animal tissue (Mouse Liver)
Ladder
2 ng 0.4 ng
Gene : GAPDH Plot : RNA 10 ng, 2 ng, 0.4 ng, 0.08 ng Gene : B-2M Plot : RNA 10 ng, 2 ng, 0.4 ng, 0.08 ng
0.08 ng
Product Size : 249 bp ABI 7500 FAST Product Size : 244bp ABI 7500 FAST
Gene : GAPDH Plot : RNA 10 ng, 2 ng, 0.4 ng, 0.08 ng
Product Size : 249 bp ABI 7500 FAST
10 ng
0.4 ng
2 ng
- Comparison of migration pattern between HiGene™ Total RNA Prep Kit (Ver 2.0) and other companies' total RNA Prep kits.
0.08 ng
10 ng Plant tissue(Leek) : 40 mg Animal tissue(Mouse Liver) : 20 mg
0.4 ng
Electrophoretic trace 2 ng
0.08 ng
[nt] Gene : B-2M Plot : RNA 10 ng, 2 ng, 0.4 ng, 0.08 ng
[nt]
Plant tissue (Leek)
Animal tissue (Mouse Liver)
Product Size : 244bp ABI 7500 FAST
Plant tissue (Leek)
Ladder Company A BIOFACT Company B Company C Company A BIOFACT Company B Company C
Gene : B-2M Plot : RNA 10 ng, 2 ng, 0.4 ng, 0.08 ng
Product Size : 244bp ABI 7500 FAST
Company A BIOFACT Company B Company C
BIOFACT
A 사
C 사
B 사
Animal tissue (Mouse Liver)
Compan BIOFACT B 사 C 사
A 사y A BIOFACT Company B Company C
Plant tissue(Leek) : 40 mg Animal tissue(Mouse Liver) : 20 mg
Plant tissue (Leek)
175
BIOFACT CATALOGUE
A 사 BIOFACT B 사 C 사
Animal tissue (Mouse Liver)
A 사 BIOFACT B 사 C 사

