Page 174 - 2022-23 BIOFACT Catalogue

Column Type
Solution Type
Magnetic Bead Type
Easy Type
HiGene™

Type Total RNA Prep Kit
Magnetic Solution Column Easy
Bead Type Type Type
Isolation of Total RNA from Various Samples

HiGene™ Total RNA Prep Kit is intended for isolation of high quality RNA from various samples such as plant, bacteria, animal tissue
and tissue cultured cell using a glass micro ber membrane spin column. It is a safe system which is designed to avoid completely
the use of toxic and hazardous reagents such as phenol/chloroform.

Feature
• Spin column type • Column binding capacity : 20 ㎍ RNA /column
• Membrane : Glass micro ber

Application
• RT, RT-PCR and qRT-PCR • Translation
• Northern blot analysis • Cloning
• RNase protection assays • Poly-A selection

Workflow

- HiGene™ Total RNA Prep Kit Prep Kit [RP101-100]

Mixing RB & Column binding &
Sample Washing Elute RNA Pure Total RNA

QC DATA
- Comparison of total RNA isolation efficiency on various sample scales

<Plant> <Animal Tissue and Cultured Cell> <Bacterium - Gram- >

M 1 2 3 M 1 2 M 1 2 3 M 1 2 3 M 1 2 3

Perilla frutescens (Leaf) Kaki persimmon Bovine liver HEK293F Cells E.coli
M : 1kb DNA Ladder (Leaf, Wax-coated) (Animal tissue) (Animal Cultured cell) M : 1kb DNA Ladder
1 : Company A M : 1kb DNA Ladder M : 1kb DNA Ladder M : 1kb DNA Ladder 1 : Company A
2 : Company B 1 : Company A 1 : Company A 1 : Company A 2 : Company B
3 : BIOFACT 2 : BIOFACT 2 : Company B 2 : Company B 3 : BIOFACT
3 : BIOFACT 3 : BIOFACT

174 BIOFACT CATALOGUE
M 1 2 3 M 1 2 3 M 1 2 3 M 1 2 3 M 1 2 3

Perilla frutescens (Leaf) Kaki persimmon Bovine Liver Bacillus sp. E.coli
M : 1kb DNA Ladder (Leaf, Wax-coated) (Animal tissue) M : 1kb DNA Ladder M : 1kb DNA Ladder
1 : 10 mg M : 1kb DNA Ladder M : 1kb DNA Ladder
1 : 1X10 7 1 : 1X10 7
2 : 50 mg 1 : 10 mg 1 : 10 mg 2 : 1X10 8 2 : 1X10 8
3 : 100 mg 2 : 50 mg 2 : 20 mg 3 : 1X10 9 3 : 1X10 9
3 : 100 mg 3 : 30 mg

* Before DNase I treatment

10 ng
2 ng 0.4 ng
0.08 ng

Gene : GAPDH Plot : RNA 10 ng, 2 ng, 0.4 ng, 0.08 ng
Product Size : 249 bp ABI 7500 FAST

10 ng
0.4 ng
2 ng
0.08 ng

Gene : B-2M Plot : RNA 10 ng, 2 ng, 0.4 ng, 0.08 ng
Product Size : 244bp ABI 7500 FAST