Page 178 - 2022-23 BIOFACT Catalogue
P. 178

- DaBead™ Plasmid Prep Kit _ 8well Pipettor [PM851S-096]









                        Cell lysis &       DNA binding            Washing              DNA elution
                       precipitation


                    - DaBead™ Plasmid Prep Kit _ APX-32P  [PM851 A-096]
                                                                                        Sample  Magnetic  DNA
                                                                                            Bead



                        1  2  3  4  5  6  1  2  3  4  5  6  1  2  3  4  5  6  1  2  3  4  5  6  1  2  3  4  5  6
                        Bead binding     Lysis bu er    Washing bu er1,2  Elution bu er   Release bead


                    - DaBead™ Plasmid Prep Kit _ 96well Press Pipettor [PM951-096, PM451-096]








                     Cell Lysis &
                    precipitation       DNA binding             Washing               DNA elution



                  QC DATA
                  - Comparison of extraction efficiency on various scales
                  <Mini Prep>                       <Midi Prep>
                     Column      Magnetic Bead             Column  Magnetic Bead      BIOFACT Magnetic Bead
                   M   1                              2                    3                  4                   5  M  M  1                          2                         3   M  M  Mini  Midi  Maxi
                          M




                  M : 1kb DNA Ladder                M : 1kb DNA Ladder           M : 1kb DNA Ladder
                  1 : BIOFACT Column Type       2 : BIOFACT       3 : Company A         1 : Company A          2 : Company B         3 : BIOFACT
                  4 : Company B      5 : Company C
                  - Magnetic Bead Type & Cell Debris Removal Syringe Filter
                                                    <Midi Prep>
                        Low Copy     High Copy
                        1                    2         1                    2         1                     2
                    M           M            M       M           M


                                                                     ※ 1% agarose gel, 2㎕ loading
                                                                     ※ Total elution volume; Mini(100 ㎕), Midi(2 ㎖), Maxi(2 ㎖)
                  M : 1kb DNA Ladder           1 : Midi           2 : Maxi  1 : Company A      2 : BIOFACT


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