Page 60 - 2022-23 BIOFACT Catalogue

- Comparison of sensitivity of 3 types of fluorescent dyes (SFCgreen® I vs SYBR® Green I vs EvaGreen™)

DCt value
Ampli cation Plot HgDNA 50 ng HgDNA 10 ng HgDNA 2 ng HgDNA 0.4 ng
16
15 SFCgreen® I 21.39 21.36 23.61 23.56 25.93 25.81 28.21 27.95
14 SFCgreen® I
13 EvaGreen™(DQ381) 23.12 23.21 25.4 25.47 27.55 27.88 29.96 30.14
12
11 EvaGreen TM SYBR® Green I(DQ383) 23.23 23.36 25.65 25.62 27.86 27.98 30.21 30.10
10 (DQ381)
9
△Rn 8
7 ΔRn value HgDNA 50 ng HgDNA 10 ng HgDNA 2 ng HgDNA 0.4 ng
6
5 [HSP89 Gene] SFCgreen® I 14.75 14.35 14.58 14.28 14.35 14.4 14.33 14.53
4
3 SYBR® Green I EvaGreen™(DQ381) 11.86 11.85 11.98 11.92 11.64 11.76 10.96 10.69
2 (DQ383)
1 SYBR® Green I(DQ383) 3.19 3.14 3.31 3.34 3.36 3.38 3.22 3.27
0
2 4 6 8 10 12 14 16 18 20 22 24 26 28 30 32 34 36 38 40
Cycle * Ampli cation e ciency : SFCgreen® I > EvaGreen™(DQ381) > SYBR® Green I(DQ383)
Ampli cation Plot
17
16 SFCgreen® I
15
14
13 EvaGreen TM DCt value HgDNA 50 ng HgDNA 10 ng HgDNA 2 ng HgDNA 0.4 ng
12 Ampli cation Plot (DQ381)
SFCgreen® I 15.94 15.86 18.18 18.44 20.47 20.5 23.14 23.13
11 16
10 15
△Rn 9 14 SFCgreen® I
8 EvaGreen™ (DQ381) 18.26 18.38 20.59 20.58 22.73 22.8 25.75 25.71
7 13
6 12 SYBR® Green I(DQ383) 18.62 18.6 20.7 20.73 22.97 22.9 25.68 25.71
5 11 [HSP89 Gene]
4 10 SYBR® Green I
3 9
2 △Rn 8 (DQ383) ΔRn value HgDNA 50 ng HgDNA 10 ng HgDNA 2 ng HgDNA 0.4 ng
1 7 EvaGreen TM
(DQ381)
0 6 SFCgreen® I 14.64 14.5 14.65 13.03 14.66 15.01 14.52 14.71
-1 5 [GAPDH Gene]
4 2 4 6 8 10 12 14 16 18 20 22 24 26 28 30 32 34 36 38 40 EvaGreen™ (DQ381) 6.82 6.94 7.23 7.08 7.15 7.09 6.83 6.74
Cycle
3 SYBR® Green I
2 (DQ383)
1 SYBR® Green I(DQ383) 1.8 1.81 1.92 1.88 1.94 1.97 1.96 1.95
0
2 4 6 8 10 12 14 16 18 20 22 24 26 28 30 32 34 36 38 40 * Ampli cation e ciency : SFCgreen® I > EvaGreen™(DQ381) > SYBR® Green I(DQ383)
Cycle
Ampli cation Plot
16
15
14 SFCgreen® I
13
12
11 Ampli cation Plot EvaGreen TM
10 17 (DQ381)
9 16 SFCgreen® I
△Rn 8 15
7 14
6 13 EvaGreen TM
5 12 [HSP89 Gene] (DQ381)
4 11
3 10 SYBR® Green I
9
△Rn
- Comparison of sensitivity of 3 types of fluorescent dyes (SFCgreen® I vs SYBR® Green I vs EvaGreen™), UDG system
(DQ383)
2 8
1 7
0 6
5 2 4 6 8 10 12 14 16 18 20 22 24 26 28 30 32 34 36 38 40
[MAPK1 Gene]
4 Cycle SYBR® Green I
3 (DQ383)
2
1
0
-1
2 4 6 8 10 12 14 16 18 20 22 24 26 28 30 32 34 36 38 40
Ampli cation Plot ΔCt value HgDNA 50 ng HgDNA 10 ng HgDNA 2 ng HgDNA 0.4 ng
Cycle
17
16 SFCgreen® I
15 SFCgreen® I 21.42 21.47 23.78 23.79 26.31 25.94 28.56 28.68
14
13 EvaGreen TM EvaGreen™(DQ381) 23.13 23.18 25.42 25.45 27.62 27.84 30.07 30.15
12 (DQ381)
11 Ampli cation Plot SYBR® Green I(DQ383) 23.33 23.28 25.71 25.57 27.76 27.8 30.12 29.93
10 16
△Rn 9 15 814 SFCgreen® I
713 ΔRn value HgDNA 50 ng HgDNA 10 ng HgDNA 2 ng HgDNA 0.4 ng
612
[HSP89 Gene] SFCgreen® I 15.96 15.91 16.07 16.21 12.82 15.84 15.43 14.5
5 11
4 10 SYBR® Green I
3 9 (DQ383)
△Rn 2 8 EvaGreen™(DQ381) 11.05 11.62 10.74 11.77 11.53 11.34 10.72 10.54
1 7 EvaGreen TM
0 6 (DQ381) SYBR® Green I(DQ383) 3.54 3.53 3.65 3.71 3.69 3.70 3.57 3.60
-1 5
[GAPDH Gene]
2 4 6 8 10 12 14 16 18 20 22 24 26 28 30 32 34 36 38 40
4 Cycle
3 * Ampli cation e ciency : SFCgreen® I > EvaGreen™(DQ381) > SYBR® Green I(DQ383)
2 SYBR® Green I
1 (DQ383)
0
2 4 6 8 10 12 14 16 18 20 22 24 26 28 30 32 34 36 38 40
Cycle
Ampli cation Plot ΔCt value HgDNA 50 ng HgDNA 10 ng HgDNA 2 ng HgDNA 0.4 ng
17
16 SFCgreen® I SFCgreen® I 20.92 21.02 23.33 23.33 25.39 25.59 27.88 27.94
15
14
13 EvaGreen TM EvaGreen™ (DQ381) 22.72 22.62 25.25 25.21 27.46 27.2 29.7 29.28
12 (DQ381)
11 SYBR® Green I(DQ383) 22.85 22.87 25.3 25.2 27.56 27.62 30.18 29.63
10
△Rn 9 8
7 ΔRn value HgDNA 50 ng HgDNA 10 ng HgDNA 2 ng HgDNA 0.4 ng
6
5 [MAPK1 Gene] SFCgreen ® I 16.37 16.12 16.29 16.35 16.14 16.02 15.68 15.57
4 SYBR® Green I
3 (DQ383)
2 EvaGreen™ (DQ381) 12.74 12.78 12.71 12.87 11.4 11.18 10.74 9.69
1
0 SYBR® Green I(DQ383) 3.52 3.57 3.58 3.67 3.56 3.62 3.36 3.40
-1
2 4 6 8 10 12 14 16 18 20 22 24 26 28 30 32 34 36 38 40
Cycle
* Ampli cation e ciency : SFCgreen® I > EvaGreen™(DQ381) > SYBR® Green I(DQ383)
60 BIOFACT CATALOGUE