Page 59 - 2022-23 BIOFACT Catalogue

DNA / RNA Amplification Real-Time PCR Enzyme

BioFACT™ Real-Time

PCR Series ⓗ (With SFCgreen® I)

Accurate and Highly Sensitive Real-Time PCR using SFCgreen® I

BioFACT™ 2X Real-time PCR Kit using SFCgreen® I contains H-Star Taq DNA polymerase with Hot Start PCR function, so that only target
DNA is speci cally ampli ed. BioFACT™ 2X Real-time PCR Kit is a pre-mixed form of H-Star Taq DNA Polymerase, dNTP, Reaction bu er
and SFCgreen® I, so that it can be conveniently used by simply adding the primer and the template to perform qPCR rection.
The use of passive reference dye di ers depending on the model, so we have produced a variety of products suitable for many models
to increase user convenience. Also, for pre-mixed products, it is available in three types: 0.1㎖ 8-strip Frosted Tube, White Tube, and Clear
Tube, so it can be used in various models.

Feature
• Highly specific amplification using • Excellent sensitivity & fluorescence against heat,
BioFACT™ H-Star Taq DNA polymerase light and chemicals
• Pre-denaturation time : 15 mins. • Suitable for UDG system

Application
• Quanti cation of target DNA using Real-Time PCR • Application using qPCR
• Quanti cation of target RNA using RT-PCR • High Resolution Melting (HRM) analysis

QC DATA

- Comparison of PCR inhibition test using fluorescent dyes between EvaGreen™ & SFCgreen® I

< EvaGreen™ > < SFCgreen® I >

Ampli cation P Ampli cation Plot Ampli cation P Ampli cation Plot
lot
lot
25.0 25.0 25.0 25.0
1x
22.5 22.5 22.5 22.5
20.0 20.0 20.0 20.0
0.75x
17.5 17.5 17.5 17.5
15.0 15.0 15.0 15.0
△Rn 12.5 △Rn 12.5 △Rn 12.5 △Rn 12.5 0.5x
10.0 10.0 1x 10.0 10.0
7.5 7.5 0.75x 7.5 7.5 0.25x
5.0 5.0 0.5x 5.0 5.0
2.5 2.5 0.25x 2.5 2.5
0.0 0.0 0.0 0.0
2 4 6 8 10 12 14 16 18 20 22 24 26 28 30 32 34 36 38 40 2 4 6 8 10 12 14 16 18 20 22 24 26 28 30 32 34 36 38 40
2 4 6 8 10 12 14 16 18 20 22 24 26 28 30 32 34 36 38 40
2 4 6 8 10 12 14 16 18 20 22 24 26 28 30 32 34 36 38 40
Cycle Cycle Cycle Cycle

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BIOFACT CATALOGUE