Page 65 - 2022-23 BIOFACT Catalogue

DNA / RNA Amplification Real-Time PCR Enzyme

BioFACT™ Real-Time

PCR Series ⓗ (With EvaGreen™)

Highly Sensitive and Accurate Real-Time PCR using EvaGreen™

- Comparison of sensitivity in various concentrations of template DNA between BioFACT™ 2X Real-Time PCR Kit
(with EvaGreen™) and other company’s Real-Time PCR kit (Limit of Detection)

△Ct Value 50 ng 10 ng 2 ng 0.4 ng
BIOFACT 18.26 21.52 25.60 29.27
Company A 21.63 24.94 28.93 32.73
BIOFACT Company A
Target : Human genomic DNA
Gene : MAPK1

Product Information
Standard Type (With EvaGreen™)
BioFACT™ Real-Time PCR Kit ⓗ [DQ191-250]
▶Contents ▶ Expiration date
EvaGreen™ (20X in water) 1 Year at -20 ± 5℃
BioFACT™ H-Star Taq DNA Polymerase (2.5 U/㎕)
10X Reaction Bu er (25 mM MgCl2 mixed)
5X Band Helper™
Each 10 mM dNTP Mix (dATP, dTTP, dGTP, dCTP)

Master Mix Type (With EvaGreen™) Master Mix Type (Including EvaGreen™)
BioFACT™ 2X Real-Time PCR Master Mix ⓗ [DQ371-40h] BioFACT™ 2X Real-Time PCR Master Mix ⓗ [DQ381-40h]

▶Contents ▶ Expiration date ▶Contents ▶ Expiration date
EvaGreen™ (20X in water) 1 Year at -20 ± 5℃ 2X Real-Time PCR Master Mix 1 Year at -20 ± 5℃
2X Real-Time PCR Master Mix (including EvaGreen™)
[1 ㎖ x 4 ea] [1 ㎖ x 4 ea]
Reference
1. Triterpene and Flavonoid Biosynthesis and Metabolic Pro ling of Hairy Roots, Adventitious Roots, and Seedling Roots of Astragalus membranaceus. Park YJ1,ThweAA1,
Li X1, Kim YJ1, Kim JK2, Arasu MV, Al-Dhabi NA, Park SU1./J Agric Food Chem. 2015 Oct 14;63(40):8862-9. doi: 10.1021/acs.jafc.5b02525. Epub 2015 Oct 1
Type Cat.No. Product Size
DQ191-250 BioFACT™ Real-Time PCR Kit with 10 mM dNTP Mix ⓗ (each 10 mM, 0.2 ㎖), 25mM MgCl2, with EvaGreen™ 500 ㎕ 250 U
Standard
DQ191-500 BioFACT™ Real-Time PCR Kit with 10 mM dNTP Mix ⓗ (each 10 mM, 0.4 ㎖), 25mM MgCl2, with EvaGreen™ 1㎖ 500 U
DQ371-40h BioFACT™ 2X Real-Time PCR Master Mix ⓗ ( nal 2.5mM MgCl2), with EvaGreen™ 500 ㎕ 1 ㎖ x 4 ea
DQ372-40h BioFACT™ 2X Real-Time PCR Master Mix ⓗ ( nal 2.5mM MgCl2), w/o EvaGreen™ 500 ㎕ 1 ㎖ x 4 ea
Master Mix
DQ381-40h BioFACT™ 2X Real-Time PCR Master Mix ⓗ ( nal 2.5mM MgCl2), including EvaGreen™ in mixture 1 ㎖ x 4 ea
DQ376-40h BioFACT™ 2X Real-Time PCR Master Mix ⓐ, including EvaGreen™ in mixture, with low Rox reference dye 1 ㎖ x 4 ea

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BIOFACT CATALOGUE