Page 66 - 2022-23 BIOFACT Catalogue

20X EvaGreen™

Intercalating Dye with High Sensitivity and Stability

20X EvaGreen™ is the next generating DNA binding dye that can be used in Real-Time PCR. EvaGreen™ is compatible with HRM
analysis as it has less PCR interference, such as slowing down the primer dimer formation and extension reaction, as well as high
sensitivity to double-stranded DNA, and can produce more accurate results. It has little PCR inhibition even at high concentrations
and is compatible with most qPCR instruments in a wavelength similar to SYBR® Green I. It is non-mutagenic, noncytotoxic, and very
stable under various PCR conditions.
Application
• λabs/λem = 500/530 nm (DNA bound) • Less PCR inhibition by dye concentration
• λabs = 471 nm (w/o DNA) • Superior bright fluorescence & Long half-life
• Similar wavelength with that for SYBR®green I or FAM • HRM / Melting curve analysis available
• Non-mutagenic, Non-cytotoxic

Comparison test between EvaGreen™ and SYBR® Green I

△Ct Value 50 ng 10 ng 2 ng 0.4 ng
EvaGreen™ 18.26 21.52 25.60 29.27
SYBR® Green I 18.27 21.18 24.97 28.78
Target : Human genomic DNA
Gene : MAPK1
EvaGreen™ SYBR® GreenⅠ

Product Information
20X EvaGreen™ [31000-B500]

▶Contents
20X EvaGreen™ [500 ㎕]
▶Expiration date
1 Year at -20 ± 5℃

Reference
1. Plant Physiol Biochem. 2015 Sep;94:19-27. doi: 10.1016/j.plaphy.2015.05.003. Epub 2015 May 9. Transgenic poplar expressing Arabidopsis YUCCA6 exhibits
auxin-overproduction phenotypes and increased tolerance to abiotic stress. Ke Q1, Wang Z2, Ji CY1, Jeong JC1, Lee HS1, Li H2, Xu B2, Deng X2, Kwak SS3.
2. Ke, Qingbo, et al. "Transgenic poplar expressing codA exhibits enhanced growth and abiotic stress tolerance." Plant Physiology and Biochemistry (2016)

Type Cat.No. Product Size
BIOFACT 31000-B500 20X EvaGreen™ 500 ㎕

66 BIOFACT CATALOGUE