Page 1306 - Clinical Immunology_ Principles and Practice ( PDFDrive )

1268 Part eleven Diagnostic Immunology

3%0&V
IURP KHDOWK\ FRQWURO

&RXQW

S%7. <

5DPRV FHOOV

&RXQW

S%7. <
$
3%0&V
IURP KHDOWK\ FRQWURO 5DPRV FHOOV

S%7. < S%7. <
%
FIG 93.13a, B Analysis of BTK Phosphorylation in PBMC and Ramos B-Cell Line. Bruton’s
tyrosine kinase (BTK) phosphorylation can be assessed in B cells in X-linked agammaglobulinemia
(XLA) patients with leaky defects who have some preserved B cells in blood. B cells are stimulated
with either anti-immunoglobulin M (IgM) (B-cell receptor [BCR] cross-linking; green line) or per-
vanadate (positive control; red line). The example shown here is for BTK phosphorylation at the
Y223 residue in peripheral blood mononuclear cells (PBMCs) from a healthy donor (top panel)
and a B-cell line, Ramos cells. The blue line represents the unstimulated control. In the bottom
panel, BTK phosphorylation of the Y551 residue is shown for PBMCs from a healthy donor (left)
and Ramos cells (right). The phosphorylation of the Y551 residue cannot be visualized for anti-IgM
stimulation, and therefore data are shown only for the positive control, pervanadate.