Page 739 - Clinical Hematology_ Theory _ Procedures ( PDFDrive )

APPENDIX G ■ The Microscope 723

Step Procedural Steps

16 Following examination of a specimen slide, remove the slide and clean the microscope by wiping off the objective

lens with lens paper. Be careful to clean the oil immersion lens last.

17 Return the low power (10×) objective to the viewing position and completely decrease the working distance by lowering

the objective to the lowest possible position.

18 Cover the microscope and return to storage, if it is not stored on the laboratory benchtop.

TIPS FOR CLEANING THE MICROSCOPE a circular motion, beginning at the center an moving out-

war . Repeat with new ampene lens paper as necessary.

Cleaning the Exterior Finally, blot ry with clean lens paper. Do not rub; this may

scratch the sur ace o the lens.
T e sur ace o most microscopes may be kept clean by wash- Lenses shoul never be touche with the f ngers.

ing it with a neutral soap an water. o clean the metal an Objectives must not be taken apart because even a slight

enamel, a gauze or so cloth shoul be moistene with alteration o the lens setting may ruin the objective. Merely

the cleaning agent an rubbe over the sur ace with a cir- clean the outer sur ace o the lens as escribe . An espe-

cular motion. T e sur ace shoul be rie imme iately cially irty objective may be unscrewe rom the nosepiece

with a Kimwipe or clean, ry piece o gauze. Kimwipes or then hel upsi e own an checke or cleanliness by using

gauze shoul never be use to clean the optical parts o the the ocular (remove rom the bo y tube) as a magni ying

microscope.
glass. Dust or lint can also be remove rom the rear lens o

the objective by blowing it away with an air syringe. Such
Cleaning Optical Lenses: General removal o the objective rom the nosepiece is not a routine

Comments cleaning proce ure. T e f nal step a er using the microscope

T e glass sur aces o the ocular, the objectives, an the con- shoul always be to wipe o all objectives with clean lens

enser are han -groun optical lenses. T ese lenses must be paper.

kept meticulously clean. Optical glass is so er than or inary

glass an shoul never be cleane with paper tissue or gauze; Cleaning the Ocular

these materials will scratch the lens. o clean the lenses o the Te ocular (eyepiece) is susceptible to irt because o its loca-

microscope, use lens paper. tion on the microscope an contact with the observer’s eye.

Be ore polishing with lens paper, take care that nothing Mascara presents a constant cleaning problem. Dust can be

is present that will scratch the optical glass in the polishing remove rom the lens o the ocular with an air syringe or

process. Such potentially abrasive irt, ust, or lint can easily camel’s hair brush; air is probably easier to use an more

be blown away be ore polishing. e cient. T e lens shoul then be polishe with lens paper.

T e ocular can be checke or a itional irt by hol ing it
Cleaning the Objectives up to a light an looking through it. When looking into the

Oil must be remove rom the oil-immersion (100×) objec- microscope, irt on any part o the ocular will rotate with the

tive imme iately a er use by wiping with clean lens paper. ocular when it is turne . T e ocular shoul not be remove

I not remove , oil may seep insi e the lens or ry on the or more than a ew minutes; ust can collect in the bo y tube

outsi e sur ace o the objective. T e high- ry (40×) objective an settle on the rear lens o an objective.

shoul never be use with oil, but i this or any other objec-

tive or microscope part comes into contact with oil, it shoul Cleaning the Condenser

be cleane imme iately. I a lens is especially irty, it may T e light source an con enser shoul also be ree o ust,

be cleane with a small amount o commercial lens cleaner, lint, an irt. First, blow away the ust with an air syringe

methanol, or manu acturer-recommen e solution applie or camel’s hair brush, then polish the light source an con-

to lens paper then wipe across the sur ace. enser with lens paper. It may be necessary to clean these

o clean the oil-immersion lens properly, f rst lower the parts urther with lens paper moistene with a commercial

stage, then rotate the objective to the ront an wipe gently lens cleaner or methanol be ore polishing them with lens

with clean lens paper. Clean o the immersion oil with lens paper.

paper ampene with special lens cleaner.

Alternatively, the cleaning agent may be applie to a Cleaning the Stage and Adjustment Knobs

woo en applicator stick wrappe with cotton or lens paper

an moistene with the cleaning agent. Do not use a plastic T e stage o the microscope shoul be cleane a er each use

applicator stick; it will be issolve by the solvent, ruining by wiping with gauze or a tissue. A er it has been cleane

the objective. Apply the cleaning agent by blotting an using thoroughly, the stage shoul be wipe ry.