Page 26 - Drug Discovery and Development: Prospects and Challenges

16 / Drug Discovery and Development: Prospects and Challenges

Table 1: Cocktail used in adipocyte differentiation study
Negative Positive Positive control 2 Treatment 1
Day
control control 1 (Rosiglitazone) (Sample X)
0 Complete Dexamethasone Dexamethasone Dexamethasone
DMEM (0.25 µM) (0.25 µM) (0.25 µM)
IBMX (0.5 mM) IBMX (0.5 mM) IBMX (0.5 mM)
Insulin (10 µg/mL) Insulin (10 µg/mL) Insulin (10 µg/mL)
Complete DMEM Rosiglitazone Sample X (10, 45,
Complete DMEM and 60 μg/ mL)
Complete DMEM
2 Complete Insulin (10 µg/mL) Insulin (10 µg/mL) Insulin (10 µg/mL)
DMEM Complete DMEM Rosiglitazone Sample X (10, 45,
Complete DMEM and 60 μg/ mL)
Complete DMEM
4 Complete Complete DMEM Complete DMEM Rosiglitazone Sample X (10, 45,
Rosiglitazone
Sample X (10, 45,
Complete DMEM
and 60 µg/ mL)
DMEM Complete DMEM and 60 μg/ mL)
Complete DMEM
4 Complete DMEM Complete DMEM Rosiglitazone Sample X (10, 45,
Complete DMEM
6 Complete Complete DMEM Rosiglitazone Complete DMEM and 60 µg/ mL)
Sample X (10, 45,
Complete DMEM
Complete DMEM
6 DMEM Complete DMEM Complete DMEM Rosiglitazone Sample X (10, 45,
and 60 μg/ mL)
Complete DMEM and 60 µg/ mL)
Complete DMEM
Sample X (10, 45,
Complete DMEM Complete DMEM
8 8 Complete Complete DMEM Rosiglitazone Rosiglitazone Complete DMEM
Sample X (10, 45,
Complete DMEM
DMEM Complete DMEM and 60 μg/ mL)
and 60 µg/ mL)
Complete DMEM
Complete DMEM

Figure 13: Simplified procedure of in vitro diabetes study using cell culture

Figure 13: Simplified procedure of in vitro diabetes study using cell culture

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