Page 59 - B.Tech Biotech Curriculum and Syllabus R2017 - REC

Department of BIOTECHNOLOGY, REC

REFERENCES
1. Anton Moser, ―Bioprocess Technology, Kinetics and Reactors‖, , Springer Verlag.
2. James E. Bailey & David F. Ollis, Biochemical Engineering Fundamentals, McGraw Hill.
3. James M. Lee, Biochemical Engineering, PHI, USA.
4. Atkinson, Handbook of Bioreactors,Harvey W. Blanch, Douglas S. Clark, Biochemical Engineering,
Marcel Decker Inc.

BT17612 GENETIC ENGINEERING LABORATORY L T P C
0 0 4 2

OBJECTIVES:
 Provide hands-on experience in performing basic recombinant DNA techniques.
 Introduce students to the theory behind in each techniques and to describe common applications of
each methodology in biological research.

EXPERIMENTS
1. Preparation of plasmid DNA
2. Elution of DNA from agarose gels
3. Ligation of DNA into expression vectors
4. Transformation
5. Optimisation of inducer concentration for recombinant protein expression
6. Optimisation of time of inducer for recombinant protein expression
7. SDS-PAGE
8. Western blotting
9. Hybridisation with anti-sera
10. PCR.
11. Preparation of RNA by Trizol method

TOTAL :60 PERIODS

COURSE OUTCOMES:
By the end of this course, students should be able to:
 Describe the main principles, methods for preparation and cloning of DNA in various organisms.
 Express clearly about the gene amplification and methods for analysis of DNA, such as hybridization,
restriction analysis and gene expressions.
 Use genetic and biotechnological techniques to manipulate genetic materials and develops new and
improved living organisms.
 Students will be aware of the hazardous chemicals and safety precautions in case of emergency.

REFERENCES
1. Old RW, Primrose SB, ―Principles Of Gene Manipulation, An Introduction To Genetic Engineering ―,
Blackwell Science Publications, 1993.
2. Ansubel FM, Brent R, Kingston RE, Moore DD, ―Current Protocols In Molecular Biology ―, Greene
Publishing Associates, NY, 1988.
3. Berger Sl, Kimmer AR, ―Methods In Enzymology‖, Vol 152, Academic Press, 1987

BT17613 NUMERICAL PROGRAMMING FOR BIOTECHNOLOGISTS
L T P C 0
0 2 1
COURSE OBJECTIVES:
 To learn the MATLAB environment and its programming fundamentals
 Ability to write Programs using commands and functions
 Able to handle polynomials, and use 2D Graphic commands
Curriculum and Syllabus | B.Tech. Biotechnology | R2017 Page 59