Page 63 - B.Tech Biotech Curriculum and Syllabus R2017 - REC

Department of BIOTECHNOLOGY, REC

UNIT IV IMMUNE TOLERANCE AND HYPERSENSITIVITY 8
Immune tolerance, Immuno deficiencies; Transplantation and graft rejection, Allergy and hypersensitivity –
Types of hypersensitivity, Autoimmunity, Auto immune disorders and diagnosis
UNIT V APPLIED IMMUNOLOGY 7
Monoclonal antibodies, engineering of antibodies; immunization and vaccine, immunodiagnostic methods
(Immuno diffusion ELISA, FACS), immuno modulatory drugs

TOTAL: 45 PERIODS
COURSE OUTCOMES:
At the end of course,
 Students will have a sound knowledge about the immune system and their role in disease protection
 Students will explain antigen antibody interaction.
 Student will gain indepth knowledge about the development of mature lymphocytes in lymphodial
organs.
 Students acquired knowledge about the immune response against various pathogen and tumor antigen
 Students acquired familiarity regarding autoimmune disorders.

TEXT BOOKS:
th
1. Roitt I, Male, Brostoff. Immunology, Mosby Publ., XII edition 2011. 52
2. Kuby J, Immunology, WH Freeman & Co., 7th Edition 2012.
3. Ashim K. Chakravarthy, Immunology, Tata McGraw-Hill, 2006.

REFERENCES:
1. Coico, Richard ―Immunology: A Short Course‖ VI Edition. John Wiley, 2008.
th
2. Khan, Fahim Halim ―Elements of Immunology‖ Pearson Education, 2009.

BT17703 PROTEIN ENGINEERING L T P C
3 0 0 3

OBJECTIVES:
At the end of the course, the student would have learnt structure and function of proteins of particular
importance, the student will know the production of recombinant insulin & in general how to engineer protein to
be used as therapeutics.

UNIT I BONDS, ENERGIES, BUILDING BLOCKS OF PROTEINS 10
Covalent, Ionic, Hydrogen, Coordinate, hydrophobic and Vander walls interactions in protein structure.
Interaction with electromagnetic radiation (radio, X-ray) and elucidation of protein structure. Amino acids
(the students should be thorough with three and single letter codes) and their molecular properties (size,
solubility, charge, pKa). Chemical reactivity in relation to post-translational modification (involving amino,
carboxyl, hydroxyl, thiol, imidazole groups).

UNIT II PROTEIN ARCHITECTURE 10
Primary structure: peptide mapping, peptide sequencing - automated Edman method & mass-spec. Secondary
structure: Alpha, beta and loop structures and methods to determine. Super-secondary structure: Alpha-turn-
alpha, beta-turn-beta (hairpin), beta-sheets, alpha-beta-alpha, topology diagrams, up and down & TIM barrel
structures nucleotide binding folds.

UNIT III TERTIARY STRUCTURE 5
Tertiary structure: Domains, folding, denaturation and renaturation, overview of methods to determine 3D
structures. Quaternary structure: of proteins.

UNIT IV STRUCTURE-FUNCTION RELATIONSHIP 10
DNA-binding proteins: prokaryotic transcription factors, Helix-turn-Helix motif in DNA binding, Trp
repressor, Eukaryotic transcription factors, Zn fingers, helix-turn helix motifs in homeodomain, Leucine
zippers, Membrane proteins: General characteristics, Trans-membrane segments, prediction,
Curriculum and Syllabus | B.Tech. Biotechnology | R2017 Page 63