Page 62 - B.Tech Biotech Curriculum and Syllabus R2017 - REC

Department of BIOTECHNOLOGY, REC

Chromatography – principles, instruments and practice, adsorption, reverse phase, ion-exchange, size
exclusion, hydrophobic interaction, bio-affinity and pseudo affinity chromatographic techniques.

UNIT V FINAL PRODUCT FORMULATION AND FINISHING OPERATIONS 5
Crystallization, drying and lyophilization in final product formulation.

TOTAL : 45 PERIODS
COURSE OUTCOMES:
Upon success completion of this course, the students will be able to:
 Students will have in depth idea about downstream processing, Pretreatment and stabilization of
bioproducts.
 Students will learn the basic principles of filtration and centrifugation.
 Students will be familiar with the fundamental ideas about Extraction and membrane separation
techniques and will learn how to develop models for precipitation technique.
 Students will be able to work on chromatographic techniques and able to do Scaling up of
chromatography.
 Students will know the fundamental concept and operational principles of Crystallization, Drying and
Lyophilization and to do research.

TEXT BOOKS:
1. Belter, P.A., E.L. Cussler and Wei-Houhu ―Bioseparations – Downstream Processing for Biotechnology‖,
John Wiley, 1988.
2. Sivasankar, B. ―Bioseparations : Principles and Techniques‖. PHI, 2005.
3. Asenjo, Juan A. ―Separation Processes in Biotechnology‖. CRC / Taylor & Francis, 1990.

REFERENCES
1. Ghosh, Raja ―Principles of Bioseparations Engineering‖. World Scientific, 2006
2. ―Product Recovery in Bioprocess Technology‖. (BIOTOL – Biotechnology by Open Learning Series).
Butterworth – Heinmann / Elsevier, 2004.

BT17702 IMMUNOLOGY L T P C
3 0 0 3

OBJECTIVES:
To discuss the structure, functions and integration of immune system.
 To explain the antigen-antibody interactions and how the immune system is protecting the body from
foreign pathogens/germs.
 To explain various techniques of monoclonal and engineered antibodies (important therapeutic
molecules) production, for treating most of the human diseases.

UNIT I INTRODUCTION TO IMMUNE SYSTEM 10
Organisation and classification of immune system – immune cells and lymphoid organs; innate and acquired
immunity; immune response (HMI & CMI), classification of antigens – chemical and molecular nature;
haptens, adjuvants; cytokines; antigen presenting cells; major histocompatibility complex

UNIT II T-CELL AND B-CELL BIOLOGY 10
Development, maturation, activation, differentiation and classification of T-cells and B-cells, antigen
processing and presentation, antibodies: structure and functions; antibodies: antigen-antibody reactions,
complement pathways and function.

UNIT III IMMUNITY AGAINST PATHOGENS AND TUMORS 10
Inflammation; protective immune responses to virus, bacteria, fungi and parasites; tumor antigens, mechanism
of tumor immune response and tumor immunotherapy

Curriculum and Syllabus | B.Tech. Biotechnology | R2017 Page 62