Page 65 - B.Tech Biotech Curriculum and Syllabus R2017 - REC

Department of BIOTECHNOLOGY, REC

COURSE OUTCOMES
Upon success completion of this course, the students will be able to:
 Students will acquire knowledge for the separation of whole cells and other insoluble ingredients
from the culture broth.
 Students get the ability to apply their knowledge for cell disruption and specify the techniques to
release intracellular products
 Students can use the techniques like evaporation, extraction, precipitation, membrane separation for
concentrating biological products.
 Students themselves able to apply the basic principles and techniques of chromatography to purify
the biological products
 Students earns broad education in formulating the product which is necessary to understand the
impact of engineering solutions in a global, economic, environmental, and societal context.

REFERENCES
1. P.A. Belter, E.L. Cussler And Wei-Houhu – Bioseparations – Downstream Processing For Biotechnology,
Wiley Interscience Pun. (1988).
2. R.O. Jenkins, (Ed.) – Product Recovery In Bioprocess Technology – Biotechnology By Open Learning
Series, Butterworth-Heinemann (1992).
3. J.C. Janson And L. Ryden, (Ed.) – Protein Purification – Principles, High Resolution Methods And
Applications, VCH Pub. 1989.

BT17712 IMMUNOLOGY LABORATORY L T P C
0 0 4 2

OBJECTIVES:
 To train the students to perform various immunology techniques and to interpret the results
 To provide basic skills and advances in the field of immunodiagnosis
 To train the students to handle the experiment animals for immunological research

EXPERIMENTS
1. Handling of animals, immunization and raising antisera
2. Identification of cells in a blood smear
3. Identification of blood group
4. Immunodiffusion & immunoelectrophoresis
5. Testing for typhoid antigens by Widal test
6. Enzyme Linked ImmunoSorbent Assay (ELISA)
7. Isolation of peripheral blood mononuclear cells
8. Coombs test
9. Haemagglutination test
10. Pregnancy test (HCG)
11. Preparation of serum and plasma from blood
12. Identification of t cells by T-cell rossetting using sheep RBC.

TOTAL: 60 PERIODS

COURSE OUTCOMES:
At the end of course,
 Students acquired ability to design the experiments in immunology research laboratories
 Students have ability to handle the animals for immunological research
 Students will have ability to draw blood from intravenous source and to separate the serum
 Students will able identify the blood group the person
 Acquired the ability to rise antiserum against protein antigens by using experimental animals
 Acquired ability to perform and interpret the result of widal test (thypoid fever )
 Students able to isolate the PBMC and characterize
Curriculum and Syllabus | B.Tech. Biotechnology | R2017 Page 65