Page 64 - B.Tech Biotech Curriculum and Syllabus R2017 - REC

Department of BIOTECHNOLOGY, REC

bacteriorhodopsin and Photosynthetic reaction center, Immunoglobulins: IgG Light chain and heavy chain

architecture, abzymes and Enzymes: Serine proteases, understanding catalytic design by engineering trypsin,
chymotrypsin and elastase, substrate-assisted catalysis.

UNIT V PROTEOMICS 10
Introduction to the concept of proteome, components of proteomics, proteomic analysis, importance of
proteomics in biological functions, protein-protein interactions and methods to study it: protein arrays, cross
linking methods, affinity methods, yeast hybrid systems and protein arrays.

TOTAL: 45 PERIODS

COURSE OUTCOMES:
 The students can identify the difference in amino acids and understand basics of protein structure
 The students will be familiar about the folds and motifs of protein structure
 The students will be able to solve the ways and means to predict the structure of proteins
 The students will be able to comprehend the structure function correlation in proteins
 The students will be aware of importance of proteomic techniques.

TEXT BOOKS:
1. Branden C. and Tooze J., ―Introduction to Protein Structured‖ 2nd Edition, Garland Publishing, 1999.
2. Creighton T.E. ―Proteins‖ 2nd Edition. W.H. Freeman, 1993.
3. Pennington, S.R and M.J. Dunn, ―Proteomics : Protein Sequence to Function‖. Viva Books, 2002
4. Liebler, ―Introduction to Proteomics‖ Humana Press, 2002.

REFERENCES
1. Voet D. and Voet G., ―Biochemistry‖. 3rd Edition. John Wiley and Sons, 2008.
2. Haggerty, Lauren M.―Protein Structure : Protein Science and Engineering‖.Nova Science Publications,
2011.
3. Williamson, Mike ―How Proteins Work‖. Garland Science, 2012.

BT17711 DOWNSTREAM PROCESSING LABORATORY L T P C
0 0 4 2

OBJECTIVES
To enable the students to
 Provide hands on training in Downstream Processing by through simple experimentation in the
laboratory. This will be a pre-requisite for project work.

Experiments
1. Solid liquid separation – centrifugation, sedimentation, flocculation
2. Cell disruption techniques – ultrasonication
3. Precipitation – ammonium sulphite precipitation
4. Ultra filtration separation
5. Aqueous two phase extraction of biologicals
6. High resolution purification – affinity chromatography
7. High resolution purification – ion exchange chromatography
8. Product polishing – drying
9. High resolution purification – gel filtration
10. HPLC
TOTAL: 60 PERIODS

Curriculum and Syllabus | B.Tech. Biotechnology | R2017 Page 64