Page 70 - B.Tech Biotech Curriculum and Syllabus R2017 - REC

Department of BIOTECHNOLOGY, REC

COURSE OUTCOMES:
At the end of the course the students will
 comprehend the abnormalities associated with metabolic pathways
 know irregularities in hormonal disorders
 learn to protect against lifestyle disorders
 interpret laboratory data for proper diagnosis of diseases
 design methods for the diagnosis and treatment of genetic diseases.

TEXT BOOKS:
1. Richard A. H, Denise R. F, Lippincott‘s Illustrated Reviews: Biochemistry, Fifth Edition - Lippincott
Williams & Wilkins
2. M.N. Chatterjee, Rane shinde, Text book of medical biochemistry Eighth Edition - JAYPEE
publications
3. Alan H. Gowenlock, Varley‘s Practical Clinical Biochemistry, Sixth Edition- CBS Publisher

REFERENCE BOOKS
1. Carl A. Burtis, David E. Bruns, Edward R. Ashwood, Tietz Fundamentals of Clinical Chemistry, 6th
Edition - Saunders Company.
2. Thomas M. Devlin, Textbook of Biochemistry with Clinical Correlations, 4th Edition - Wiley and
Sons Ltd. 1997.
3. Salway, J.G., ―Metabolism at a Glance‖. IInd Edition, Blackwell Science Ltd., 2000.

BT17E61 ANIMAL BIOTECHNOLOGY L T P C
3 0 0 3
OBJECTIVES:
 To develop the skills of the students in the area of animal biotechnology and its applications
 The student will learn about animal cell culture, molecular diagnostic of animal diseases and
Transgenic animal production.
 This subject will facilitate the student to undertake project work in this area.

UNIT I ANIMAL CELL CULTURE 12
Basic tissue culture techniques, animal cell culture media – types, advantages and disadvantages of serum
containing and serum free media. Animal cell cultures – primary culture, secondary culture, maintenance and
preservation. Various types of cultures – mono layers, suspension cultures, continuous flow cultures,
immobilized cultures. Somatic cell fusion, cell cultures as a source of valuable products and organ cultures.

UNIT II ANIMAL DISEASES AND THEIR DIAGNOSIS 10
Bacterial and viral diseases in animals; monoclonal antibodies and their use in diagnosis; molecular diagnostic
techniques like PCR, in-situ hybridization; northern and southern blotting; RFLP.

UNIT III THERAPY OF ANIMAL DISEASES 12
Recombinant cytokines and their use in the treatment of animal infections; monoclonal antibodies in therapy;
vaccines and their applications in animal infections.

UNIT IV MICROMANIPULATION OF EMBRYO’S 6
Micromanipulation technology – definition and equipments used in micromanipulation. Enrichment of x and
y bearing sperms from semen samples of animals; artificial insemination and germ cell manipulations; in vitro
fertilization and embryo transfer; micromanipulation technology and breeding of farm animals.

UNIT V TRANSGENIC ANIMALS 5
Concepts of transgenic animal technology; strategies for the production of transgenic animals and their
importance in biotechnology; stem cell cultures in the production of transgenic animals.

TOTAL: 45 PERIODS

Curriculum and Syllabus | B.Tech. Biotechnology | R2017 Page 70