Page 72 - B.Tech Biotech Curriculum and Syllabus R2017 - REC

Department of BIOTECHNOLOGY, REC

 To study the gene expression networks.
 To work on different tools of Systems Biology and application.
TEXT BOOKS
1. EddaKlipp, Wolfram Liebermeister, ChristophWierling Axel Kowald, Systems Biology: A
Textbook,Willey,2016
2. Hans Westerhoff (Editor), MalkheyVerma (Editor), Daniel Jameson. Methods in Systems Biology.

REFERENCES
1. AlpanRaval,Animesh Ray. Introduction to Biological Networks.Taylor and Francis Group. 2013.

BT17E63 BIOLOGICAL SPECTROSCOPY L T P C
3 0 0 3

OBJECTIVES:
 To deliver the knowledge of spectroscopic techniques and its functions
 To provide the technical information of spectroscopy for biological applications

UNIT I OPTICAL ROTATORY DISPERSION 5
Polarized light – optical rotation – circular dichroism – circular dichroism of nucleic acids and proteins.

UNIT II NUCLEAR MAGNETIC RESONANCE 10
Chemical shifts – spin – spin coupling – relaxation mechanisms – nuclear overhauser effect–
multidimensional nmr spectroscopy – determination of macromolecular structure by NMR – magnetic
resonance imaging.

UNIT III MASS SPECTROMETRY 10
Ion sources sample introduction – mass analyzers and ion detectors – bimolecular mass spectrometry –
peptide and protein analysis – carbohydrates and small molecules – specific applications.

UNIT IV X-RAY DIFFRACTION 10
Scattering by x- rays – diffraction by a crystal – measuring diffraction pattern – Bragg reflection – unit cell –
phase problem – anomalous diffraction – determination of crystal structure – electron and neutron diffraction.

UNIT V SPECIAL TOPICS AND APPLICATIONS 10
Electron microscopy – transmission and scanning electron microscopy – scanning tunneling and atomic force
microscopy – combinatorial chemistry and high throughput screening methods.

TOTAL : 45 PERIODS

COURSE OUTCOMES:
Upon completion of this course, the student would be able understand
 Basics of optical rotary dispersion methods
 Basics of nuclear magnetic resonance
 Principles and applications of mass spectrometry
 Ideologies of X-ray diffraction About the microscopic techniques and applications
 Apply the spectroscopic techniques for various biological applications

TEXT BOOKS:
1. Hammes G.G., ―Spectroscopy For The Biological Sciences‖, Wiley-Inter Science, First Edition, 2005.
2. Ramamoorthy A., ―NMR Spectroscopy Of Biological Solids‖, CRC Press, 2005.

REFERENCES:
1. Campbell I.D And Dwek R.A., ― Biological Spectroscopy ―, Benjami Cummins And
Company, 1986.
Curriculum and Syllabus | B.Tech. Biotechnology | R2017 Page 72