Page 62 - 2022-23 BIOFACT Catalogue

BioFACT™ Real-Time

PCR Series ⓐ (With SFCgreen® I)

Accurate and Rapid Real-Time PCR Series using SFCgreen® I

BioFACT™ 2X Real-time PCR Kit using SFCgreen® I contains A-Star Taq DNA polymerase with hot start PCR function, so that only
target DNA is speci cally ampli ed. BioFACT™ A-Star 2X Real-time PCR Kit is a pre-mixed type of A-Star Taq DNA Polymerase, dNTP,
Reaction bu er and SFCgreen® I, so that it can be conveniently used by simply adding the primer and the template to perform
qPCR reaction. The use of passive reference dye di ers depending on the model, so we have produced a variety of products suita-
ble for many models to increase user convenience. Also, for pre-mixed products, it is available in three types: 0.1㎖ 8-strip Frosted
Tube, White Tube, and Clear Tube, so it can be used in various models.

Feature
• Highly specific amplification using • Excellent sensitivity & fluorescence against heat,
BioFACT™ A-Star Taq DNA polymerase light and chemicals
• Pre-denaturation time : 2 mins. • Suitable for UDG system

Application
• Quanti cation of target DNA using Real-Time PCR • Application using qPCR
• Quanti cation of target RNA using RT-PCR • High resolution melting (HRM) analysis

QC DATA
- Comparison of PCR inhibition test using fluorescent dyes between EvaGreen™ & SFCgreen® I
A-Star Taq/dNTP(T) System
< EvaGreen™ > < SFCgreen® I >
Ampli cation Plot Ampli cation Plot
30.0 30.0
27.5 27.5 1X
25.0 25.0
22.5 22.5
1X
20.0 20.0 0.75X
0.75X
17.5 17.5
△Rn 15.0 △Rn 15.0
12.5 12.5 0.5X
10.0 0.5X 10.0
7.5 [MAPK1 Gene] 7.5 0.25X
5.0 0.25X 5.0
2.5 2.5
0.0 0.0
2 4 6 8 10 12 14 16 18 20 22 24 26 28 30 32 34 36 38 40 2 4 6 8 10 12 14 16 18 20 22
Cycle Cycle
Ampli cation Plot Ampli cation Plot
30.0 30.0
27.5 27.5 1X
25.0 25.0
22.5 22.5
0.75X
20.0 20.0
17.5 17.5
△Rn 15.0 △Rn 15.0 0.5X
12.5 12.5
10.0 1X 10.0
7.5 [GAPDH Gene] 0.75X 7.5 0.25X
0.5X
5.0 5.0
2.5 0.25X 2.5
0.0 0.0
2 4 6 8 10 12 14 16 18 20 22 24 26 28 30 32 34 36 38 40 2 4 6 8 10 12 14 16 18 20 22
Cycle Cycle
62 BIOFACT CATALOGUE