Page 63 - 2022-23 BIOFACT Catalogue

DNA / RNA Amplification Real-Time PCR Enzyme

- Comparison of PCR inhibition test using fluorescent dyes between EvaGreen™ & SFCgreen® I, UDG system
A-Star Taq/dNTP(U) System
< EvaGreen™ > < SFCgreen® I >
Ampli cation Plot Ampli cation Plot
30.0 30.0 1X
27.5 27.5
25.0 1X 25.0
22.5 22.5
0.75X
20.0 20.0
17.5 0.75X 17.5
△Rn 15.0 △Rn 15.0 0.5X
12.5 0.5X 12.5
10.0 10.0
7.5 [MAPK1 Gene] 0.25X 7.5 0.25X
5.0 5.0
2.5 2.5
0.0 0.0
2 4 6 8 10 12 14 16 18 20 22 24 26 28 30 32 34 36 38 40 2 4 6 8 10 12 14 16 18 20 22
Cycle Cycle
Ampli cation Plot Ampli cation Plot
30.0 30.0
27.5 27.5 1X
25.0 25.0
22.5 22.5
0.75X
20.0 20.0
17.5 17.5
△Rn 15.0 △Rn 15.0 0.5X
12.5 12.5
10.0 1X 10.0
7.5 [GAPDH Gene] 7.5 0.25X
0.75X
5.0 0.5X 5.0
2.5 0.25X 2.5
0.0 0.0
2 4 6 8 10 12 14 16 18 20 22 24 26 28 30 32 34 36 38 40 2 4 6 8 10 12 14 16 18 20 22
Cycle Cycle
Product Information
Master Mix Type (Including SFCgreen® I ) Pre-Mix Type (Including SFCgreen® I)
BioFACT™ 2X Real-Time PCR Master Mix ⓐ [DQ352-40h] BioFACT™ 2X Real-Time PCR Pre-Mix ⓐ [DQ452-096(W)]

▶Contents ▶ Expiration date ▶Contents ▶ Expiration date
2X Real-Time PCR Master Mix 1 Year at -20 ± 5℃ 2X Real-Time PCR Pre-Mix 1 Year at -20 ± 5℃
(including SFCgreen® I) (including SFCgreen® I)
[1 ㎖ x 4 ea] [96Tubes (8strip × 12)]

Type Cat.No. Product Size
DQ352-40h BioFACT™ 2X Real-Time PCR Master Mix ⓐ, including SFCgreen® I in mixture, Low Rox reference dye 1 ㎖ × 4 ea
Master DQ353-40h BioFACT™ 2X Real-Time PCR Master Mix ⓐ, including SFCgreen® I in mixture, High Rox reference dye 1 ㎖ × 4 ea
Mix DQ357-40h BioFACT™ 2X Real-time PCR Master Mix ⓐ, including SFCgreen® I in mixture, Low Rox reference dye (UDG System) 1 ㎖ × 4 ea
DQ358-40h BioFACT™ 2X Real-Time PCR Master Mix ⓐ, including SFCgreen® I in mixture, High Rox reference dye (UDG System) 1 ㎖ × 4 ea
DQ452-096(W/F/C) BioFACT™ 2X Real-Time PCR Pre-Mix ⓐ, including SFCgreen® I in mixture, Low Rox reference dye 96Tubes (8strip × 12)
DQ453-096(F) BioFACT™ 2X Real-Time PCR Pre-Mix ⓐ, including SFCgreen® I in mixture, High Rox reference dye 96Tubes (8strip × 12)
Pre-Mix
DQ457-096(W/F/C) BioFACT™ 2X Real-Time PCR Pre-Mix ⓐ, including SFCgreen® I in mixture, Low Rox reference dye (UDG System) 96Tubes (8strip × 12)
DQ458-096(F) BioFACT™ 2X Real-Time PCR Pre-Mix ⓐ, including SFCgreen® I in mixture, High Rox reference dye (UDG System) 96Tubes (8strip × 12)
* The products containing low ROX allow choosing from White Tube(W), Frosted Tube(F), or Clear Tube(C).
* The product containing high ROX is sold with Frosted Tube (F) type only.

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BIOFACT CATALOGUE